Literature summary for 3.4.21.114 extracted from

Tian, X.; Lu, G.; Gao, F.; Peng, H.; Feng, Y.; Ma, G.; Bartlam, M.; Tian, K.; Yan, J.; Hilgenfeld, R.; Gao, G.F.
Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) (2009), J. Mol. Biol., 392, 977-993.

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Cloned(Commentary)

Cloned (Comment)	Organism
GST fusion protein expressed in Escherichia coli BL21(DE3)	porcine reproductive and respiratory syndrome virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	porcine reproductive and respiratory syndrome virus

Protein Variants

Protein Variants	Comment	Organism
S118A	catalytic residue, mutant protein inactive	porcine reproductive and respiratory syndrome virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.24	-	dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Glu-EDANS	-	porcine reproductive and respiratory syndrome virus

Organism

Organism	UniProt	Comment	Textmining
porcine reproductive and respiratory syndrome virus	A1E8J1	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione-Sepharose affinity chromatography, gel filtration	porcine reproductive and respiratory syndrome virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Glu-EDANS + H2O	-	porcine reproductive and respiratory syndrome virus	Dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Glu-EDANS	-	?
Lys-Asp-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Thr-Trp + H2O	-	porcine reproductive and respiratory syndrome virus	Lys-Asp-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Thr-Trp	-	?
Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Thr-Trp-Tyr-Gln + H2O	-	porcine reproductive and respiratory syndrome virus	Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Thr-Trp-Tyr-Gln	-	?

Subunits

Subunits	Comment	Organism
monomer	in solution	porcine reproductive and respiratory syndrome virus

Synonyms

Synonyms	Comment	Organism
3C-like serine protease	-	porcine reproductive and respiratory syndrome virus
3CLSP	-	porcine reproductive and respiratory syndrome virus
nonstructural protein 4	-	porcine reproductive and respiratory syndrome virus
nsp4	-	porcine reproductive and respiratory syndrome virus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0073	-	dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Glu-EDANS	-	porcine reproductive and respiratory syndrome virus

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Release 2023.1 (January 2023)