Cloned (Comment) | Organism |
---|---|
transient expression of wild-type and mutant enzymes in enzyme-deficient SRD12B cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H249A | site-directed mutagenesis, catalytically inactive mutant showing no autoprocessing | Homo sapiens |
Y285A | site-directed mutagenesis, molecular modeling | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
Ac-QKSIAVGRTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0028 | - |
Ac-YISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0047 | - |
Ac-IYISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.005 | - |
Ac-YISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0056 | - |
Ac-YISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0057 | - |
Ac-YISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0074 | - |
Ac-IYISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0123 | - |
Ac-FISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.015 | - |
Ac-FISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0153 | - |
Ac-RRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0156 | - |
Ac-QKSIAVGRTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0212 | - |
Ac-YSSVSRKLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0226 | - |
Ac-IAVGRTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0228 | - |
Ac-RRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0274 | - |
Ac-RKLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0281 | - |
Ac-YSSVSRKLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0443 | - |
Ac-SFITRRLQ-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0504 | - |
Ac-AISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0564 | - |
Ac-RKLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.067 | - |
Ac-AISRRLL-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0784 | - |
Ac-SFITRRLQ-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0846 | - |
Ac-IAVGRTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.0967 | - |
Ac-RTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.0971 | - |
Ac-RRLQ-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.2167 | - |
Ac-RTLK-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens | |
0.2263 | - |
Ac-RRLQ-7-amido-4-methylcoumarin | pH and temperature not specified in the publication, recombinant mutant Y285A | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-AISRRLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-AISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-FISRRLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-FISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-IAVGRTLK-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-IAVGRTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-IYISRRLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-IYISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-QKSIAVGRTLK-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-QKSIAVGRTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-RKLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-RKLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-RRLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-RRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-RRLQ-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-RRLQ + 7-amino-4-methylcoumarin | - |
? | |
Ac-RTLK-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-RTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-SFITRRLQ-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-SFITRRLQ + 7-amino-4-methylcoumarin | - |
? | |
Ac-YISRRLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-YISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-YSSVSRKLL-7-amido-4-methylcoumarin + H2O | Homo sapiens | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Ac-YSSVSRKLL + 7-amino-4-methylcoumarin | - |
? | |
arenavirus envelope glycoprotein precursor + H2O | Homo sapiens | activation, F259A replacement at P7 of the viral protein substrate impaires the reaction, while replacement of with F does not affect enzyme processing. The catalytic pocket of the enzyme may interact with additional substrate residues distal from the actual cleavage site. Residue Y285 of the enzyme, located distal from the catalytic triad, is implicated in the molecular recognition of the aromatic signature residue at P7 and is crucial for efficient processing of OW and clade C NW arenavirus glycoprotein precursor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q14703 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-AISRRLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-AISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-FISRRLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-FISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-IAVGRTLK-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-IAVGRTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-IYISRRLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-IYISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-QKSIAVGRTLK-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-QKSIAVGRTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-RKLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-RKLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-RRLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-RRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-RRLQ-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-RRLQ + 7-amino-4-methylcoumarin | - |
? | |
Ac-RTLK-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-RTLK + 7-amino-4-methylcoumarin | - |
? | |
Ac-SFITRRLQ-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-SFITRRLQ + 7-amino-4-methylcoumarin | - |
? | |
Ac-YISRRLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-YISRRLL + 7-amino-4-methylcoumarin | - |
? | |
Ac-YSSVSRKLL-7-amido-4-methylcoumarin + H2O | an arenavirus envelope glycoprotein precursor protein-derived peptide substrate | Homo sapiens | Ac-YSSVSRKLL + 7-amino-4-methylcoumarin | - |
? | |
arenavirus envelope glycoprotein precursor + H2O | activation, F259A replacement at P7 of the viral protein substrate impaires the reaction, while replacement of with F does not affect enzyme processing. The catalytic pocket of the enzyme may interact with additional substrate residues distal from the actual cleavage site. Residue Y285 of the enzyme, located distal from the catalytic triad, is implicated in the molecular recognition of the aromatic signature residue at P7 and is crucial for efficient processing of OW and clade C NW arenavirus glycoprotein precursor | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SKI-1/S1P | - |
Homo sapiens |
subtilisin kexin isozyme 1/site 1 protease | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | modeling of the SKI-1/S1P catalytic pocket identifies Y285 as a residue that potentially interacts with arenavirus envelope glycoprotein precursor residue Y253. The the catalytic triad is formed by residues D218, H249, and S414 | Homo sapiens |
physiological function | the arenaviruses are an important family of emerging viruses that includes several causative agents of severe hemorrhagic fevers in humans that represent serious public health problems. A crucial step of the arenavirus life cycle is maturation of the envelope glycoprotein precursor by the cellular subtilisin kexin isozyme 1/site 1 protease | Homo sapiens |