Literature summary for 3.4.21.111 extracted from

Tanaka, T.; Kikuchi, Y.; Matsuzawa, H.; Ohta, T.
Application of a metal switch to aqualysin I, a subtilisin-type bacterial serine protease, to the S3 site residues, Ser102 and Gly131 (2000), Biosci. Biotechnol. Biochem., 64, 2008-2011.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermus aquaticus

Protein Variants

Protein Variants	Comment	Organism
S102H/G131H	enzyme can be inhibited by metal ions	Thermus aquaticus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	inhibits S102H/G131H mutant at 0.76 mM, inhibition can be restored by addition of EDTA	Thermus aquaticus
Ni2+	inhibits S102H/G131H mutant at 0.76 mM, inhibition can be restored by addition of EDTA	Thermus aquaticus
Zn2+	inhibits S102H/G131H mutant at 0.76 mM, inhibition can be restored by addition of EDTA	Thermus aquaticus

Organism

Organism	UniProt	Comment	Textmining
Thermus aquaticus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O	-	Thermus aquaticus	N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline	-	?

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Release 2023.1 (January 2023)