Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
S512A | no autocatalytic cleavage | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition at 5 mM | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
SQLRANISHKDMQLGR | pH 7.4, 25°C | Streptococcus pyogenes | |
0.936 | - |
VVASQLRANISH | pH 7.4, 25°C | Streptococcus pyogenes | |
2 | - |
Ac-SQLRANISH-4-nitroanilide | pH 7.4, 25°C, Km above 2 mM | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chemotaxin C5a | Streptococcus pyogenes | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | propeptide is cleaved either via an autocatalytic intramolecular cleavage or by exogenous protease streotopain | Streptococcus pyogenes |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-SQLRANISH-4-nitroanilide + H2O | weak chromogenic substrate | Streptococcus pyogenes | ? | - |
? | |
chemotaxin C5a | - |
Streptococcus pyogenes | ? | - |
? | |
chemotaxin C5a + H2O | cleaves between His67 and Lys68 or Ala58 and Ser59 | Streptococcus pyogenes | ? | - |
? | |
SQLRANISHKDMQLGR + H2O | good substrate | Streptococcus pyogenes | ? | - |
? | |
VVASQLRANISH + H2O | - |
Streptococcus pyogenes | VVA + SQLRANISH | - |
? | |
VVASQLRANISHKDMQLGR + H2O | - |
Streptococcus pyogenes | VVASQLRANISH + KDMQLGR | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 43 | - |
Streptococcus pyogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 43 | increase of temperature from 5°C to 43°C results in 10fold increase in enzyme activity | Streptococcus pyogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
complete inactivation | Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
VVASQLRANISH | pH 7.4, 25°C | Streptococcus pyogenes | |
1.1 | - |
SQLRANISHKDMQLGR | pH 7.4, 25°C | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
SQLRANISHKDMQLGR as substrate | Streptococcus pyogenes |
8.6 | - |
activity against chromogenic substrate Ac-SQLRANISH-4-nitroanilide is 60% higher than at pH 7.4 | Streptococcus pyogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
activity against chromogenic substrate Ac-SQLRANISH-4-nitroanilide is 60% higher than at pH 7.4 | Streptococcus pyogenes |