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Literature summary for 3.4.21.109 extracted from

  • Zhao, N.; Nizzi, C.P.; Anderson, S.A.; Wang, J.; Ueno, A.; Tsukamoto, H.; Eisenstein, R.S.; Enns, C.A.; Zhang, A.S.
    Low intracellular iron increases the stability of matriptase-2 (2015), J. Biol. Chem., 290, 4432-4446.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.109

Cloned(Commentary)

Cloned (Comment) Organism
gene TMPRSS6, expression of C-terminally c-Myc-tagged wild-type and truncated mutant enzymes in Hep-G2 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information costruction of enzyme mutants with deletion of first 9 amino acids or the first 46 amino acids Homo sapiens

General Stability

General Stability Organism
low intracellular iron increases the stability of matriptase-2, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the cytoplasmic domain of the enzyme contains an endocytosis motif that mediates the internalization of cell surface enzyme in a dynamin-dependent manner, Iron depletion does not affect the internalization of cell surface enzyme Homo sapiens 9986
-
membrane a type II transmembrane serine protease Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information low intracellular iron increases the stability of matriptase-2, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hemojuvelin + H2O Homo sapiens a glycosylphosphatidylinositol-linked membrane protein. inactivation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IU80 gene TMPRSS6
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte predominantly Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hemojuvelin + H2O inactivation Homo sapiens ?
-
 ?
hemojuvelin + H2O a glycosylphosphatidylinositol-linked membrane protein. inactivation Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More the type II transmembrane protease is composed of a short cytoplasmic domain, a transmembrane domain, and a large extracellular domain, which contains a membrane-proximal stem region, a predicted activation domain, and a C-terminal catalytic domain Homo sapiens

Synonyms

Synonyms Comment Organism
matriptase-2
-
 Homo sapiens
MT2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens in Hep-G2 cells stably expressing the coding sequence of gene TMPRSS6, encoding the enzyme, incubation with apo-transferrin or the membrane-impermeable iron chelator deferoxamine mesylate salt, increases enzyme levels. This increase does not result from the inhibition of enzyme shedding from the cells additional information
Homo sapiens the enzyme is up-regulated under iron deprivation up

General Information

General Information Comment Organism
malfunction lack of the putative endocytosis motif in the cytoplasmic domain largely abolishes the sensitivity of the enzyme to iron depletion Homo sapiens
additional information in Hep-G2 cells stably expressing the coding sequence of gene TMPRSS6, encoding the enzyme, incubation with apo-transferrin or the membrane-impermeable iron chelator deferoxamine mesylate salt, increases enzyme levels. This increase does not result from the inhibition of enzyme shedding from the cells Homo sapiens
physiological function the enzyme suppresses the expression of hepatic hepcidin, an iron regulatory hormone, by cleaving membrane hemojuvelin into an inactive form. Hemojuvelin is a bone morphogenetic protein coreceptor. Regulation of the enzyme occurs at the level of protein degradation rather than by changes in the rate of internalization and translational or transcriptional mechanisms, the cytoplasmic enzyme domain is necessary for its regulation Homo sapiens