Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F303W | mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation | Homo sapiens |
F331W | mutation in proease domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation | Homo sapiens |
F331Y | inactive | Homo sapiens |
I329N | inactive | Homo sapiens |
L367W | mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation | Homo sapiens |
L377W | mutation in PDZ domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation | Homo sapiens |
R432L | mutation in PDZ domain, significant increase in activity | Homo sapiens |
V226K | mutation in protease domain, significant increase in activity | Homo sapiens |
V226K E429L | salt link formation is no longer possible, the increase of activity observed for mutant V226K ceased to exist | Homo sapiens |
V226W | mutation in proease domain, proteolytic activity similar to wild-type | Homo sapiens |
V325D | inactive | Homo sapiens |
V364W | mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation | Homo sapiens |
Y361W | mutation in proease domain, proteolytic activity similar to wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00158 | - |
(7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide | pH 8.0, 45°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43464 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HtrA2(Omi) | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
activity increases about 14fold between 25 and 45 °C | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
activity is practically negligible at 20 °C | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
upon an increase in temperature the HtrA2 structure relaxes, the PDZ-protease interface becomes more exposed to the solvent, and significant conformational changes involving both domains occur at and above 30 °C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide | pH 8.0, 45°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.13 | - |
(7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide | pH 8.0, 45°C | Homo sapiens |