Literature summary for 3.4.21.108 extracted from

Yang, L.; Sun, M.; Sun, X.M.; Cheng, G.Z.; Nicosia, S.V.; Cheng, J.Q.
Akt attenuation of the serine protease activity of HtrA2/Omi through phosphorylation of serine 212 (2007), J. Biol. Chem., 282, 10981-10987.

Search for more literature for 3.4.21.108

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	serine/threonine kinases Akt1 and Akt2 phosphorylate mitochondria-released HtrA2/Omi on serine 212 in vivo and in vitro, which results in attenuation of its serine protease activity and pro-apoptotic function. Akt phosphorylation of HtrA2/Omi at serine 212 is a critical event for attenuation of HtrA2/Omi cleaving X-linked inhibitor of apoptosis protein, although it does not affect HtrA2/Omi-X-linked inhibitor of apoptosis protein complex formation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
HtrA2/Omi cDNA cloned into pEGFP-C3 at KpnI/XbaI sites. HeLa cells transfected with GFP-fused premature or mature HtrA2/Omi together with or without wild-type and constitutively active Akt. HEK-293 cells transfected with pcDNA3-Myc-HtrA2/Omi together with or without wild-type and constitutively active Akt	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S212A	mutant with abolished HtrA2/Omi phosphorylation by Akt. It retains its serine protease activity and induces more apoptosis as compared with wild-type HtrA2/Omi	Homo sapiens
S212D	mutant mimicking phosphorylation. It has lost the protease activity and fails to induce programmed cell death	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Akt abrogates HtrA2/Omi pro-apoptotic function through phosphorylation of serine 212 in cytoplasm and inhibition of its release from the mitochondria in response to cisplatin treatment. Caspase inhibitor z-VAD-FMK reduces the mature HtrA2/Omi-induced cell death by ca. 45%	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-
mitochondrion	-	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
OV2008 cell	-	Homo sapiens	-
OVCAR-3 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-casein + H2O	-	Homo sapiens	?	-	?
X-linked inhibitor of apoptosis protein + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
HtrA2/Omi	-	Homo sapiens
serine protease HtrA2/Omi	-	Homo sapiens

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Release 2023.1 (January 2023)