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Literature summary for 3.4.21.107 extracted from
- Properties of the HtrA protease from bacterium Helicobacter pylori whose activity is indispensable for growth under stress conditions (2019), Front. Microbiol., 10, 961 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Helicobacter pylori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S221A | loss of catalytic activity | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | Q9ZM18 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme. Comparison of the protein from Helicobacter pylori strains 26695, J99 and N6 | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-casein + H2O | - |
Helicobacter pylori | ? | - |
? |  |
| additional information | enzyme cuts preferentially after valine at P1, with serine, leucine and isoleucine in decreasing order of preference. No substrate: native lysozyme | Helicobacter pylori | ? | - |
? | |
| reduced lysozyme + H2O | - |
Helicobacter pylori | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Helicobacter pylori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
melting temperature above 85°C, melting temepratures at pH 5.5, 6.5, or 8.0 are similar | Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Helicobacter pylori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Helicobacter pylori |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | a HtrA knockout strain shows increased sensitivity to stress such as elevated temperature, pH and osmotic shock, as well as treatment with puromycin | Helicobacter pylori |
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