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Literature summary for 3.4.21.107 extracted from

  • Cassone, M.; Gagne, A.L.; Spruce, L.A.; Seeholzer, S.H.; Sebert, M.E.
    The HtrA protease from Streptococcus pneumoniae digests both denatured proteins and the competence-stimulating peptide (2012), J. Biol. Chem., 287, 38449-38459.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
S234A catalytic site variant Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000247
-
 CSP-1 FRET peptide pH 7.4, 37°C Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Streptococcus pneumoniae ?
-
 ?
competence-stimulating peptide CSP-1 + H2O enzyme HtrA constitutes the primary extracytoplasmic competence-stimulating peptide-degrading activity in cultures of Streptococcus pneumoniae. Both substrate isoforms CSP-1 and CSP-2 interact with HtrA with similar efficiencies Streptococcus pneumoniae ? cleavage predominantly follows residue Phe8 of the CSP-1 isoform of the peptide within its central hydrophobic patch ?
competence-stimulating peptide CSP-2 + H2O enzyme HtrA constitutes the primary extracytoplasmic competence-stimulating peptide-degrading activity in cultures of Streptococcus pneumoniae. Both substrate isoforms CSP-1 and CSP-2 interact with HtrA with similar efficiencies Streptococcus pneumoniae ?
-
 ?
CSP-1 FRET peptide + H2O reporter peptide with incorporation of a QSY-7 quencher and a Cys(Alexa488) fluorophore at theN-erminus andC-terminus of CSP-1, respectively Streptococcus pneumoniae ?
-
 ?
additional information enzyme displays a preference for substrates with non-polar residues at the P1 site Streptococcus pneumoniae ?
-
 ?