Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | only when incubated in the presence of 20 mM dithiothreitol, which reduces the structural disulfide bonds and unfold the protein, and above 34°C, is CtHtrA able to proteolyse alpha-lactalbumin | Chlamydia trachomatis |
Cloned (Comment) | Organism |
---|---|
gene htrA, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) using a vector containing pelB secretion signal sequence | Chlamydia trachomatis |
Protein Variants | Comment | Organism |
---|---|---|
S247A | proteolytically inactive mutant | Chlamydia trachomatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Chlamydia trachomatis | |
additional information | no inhibition by aprotinin and trypsin inhibitor | Chlamydia trachomatis | |
PMSF | - |
Chlamydia trachomatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | activates at 20 mM | Chlamydia trachomatis | |
MgSO4 | activates at 20 mM | Chlamydia trachomatis | |
NaCl | activates at 20 mM | Chlamydia trachomatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
6 * 50000, SDS-PAGE | Chlamydia trachomatis |
307000 | - |
recombinant enzyme, gel filtration | Chlamydia trachomatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
insulin beta-chain + H2O | Chlamydia trachomatis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia trachomatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-lactalbumin + H2O | only when incubated in the presence of 20 mM dithiothreitol, which reduces the structural disulfide bonds and unfold the protein, and above 34°C, is CtHtrA able to proteolyse alpha-lactalbumin | Chlamydia trachomatis | ? | - |
? | |
beta-casein + H2O | the cleavage activity at 37°C is ATP independent, not significantly influenced by buffer composition, active over a broad range of pH, and with a broad optimum at pH 6.5, and 20 mM salts, e.g. magnesium sulfate, magnesium chloride, or sodium chloride, improving activity, determination of cleavage sites | Chlamydia trachomatis | beta-casein peptide fragments | - |
? | |
insulin beta-chain + H2O | - |
Chlamydia trachomatis | ? | - |
? | |
additional information | HtrA sequence specificity, overview, no activity with albumin and myoglobin at 37°C, HtrA acts as both a chaperone and protease, HtrA mutant S247A is able to chaperone insulin B-chain, irrespective of temperature, but at 30°C only HtrA and not mutant S247A displays significant chaperone activity for alpha-lactalbumin, overview | Chlamydia trachomatis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 50000, SDS-PAGE | Chlamydia trachomatis |
More | secondary structures for activated protease at different temperatures | Chlamydia trachomatis |
Synonyms | Comment | Organism |
---|---|---|
HtrA protease | - |
Chlamydia trachomatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, chaperone and protease activities | Chlamydia trachomatis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | 55 | low activity below 34°C, rapid loss of activity at 55°C | Chlamydia trachomatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
broad optimum | Chlamydia trachomatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Chlamydia trachomatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | ATP is not required | Chlamydia trachomatis |