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Literature summary for 3.4.21.107 extracted from
- The N-terminal region of HtrA heat shock protease from Escherichia coli is essential for stabilization of HtrA primary structure and maintaining of its oligomeric structure (2003), Biochim. Biophys. Acta, 1649, 171-182.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C57S/C69S | mutant enzyme is less stable, in contrast to wild-type enzyme the mutant protein is autocleaved even without reducing agents | Escherichia coli |
| S210A | proteolytically inactive | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| enzyme cleaves itself under reducing conditions in the presence of 2-mercaptoethanol or dithiothreitol | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
6 * 44000, gel filtration, SDS-PAGE | Escherichia coli |
| 200000 | - |
above, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | involved in the degradation of damaged proteins | ? | - |
? |  |
| Protein + H2O | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-casein + H2O | - |
Escherichia coli | ? | - |
? | |
| Lysozyme + H2O | can only be digested in the presence of reducing agents | Escherichia coli | ? | - |
? | |
| additional information | involved in the degradation of damaged proteins | Escherichia coli | ? | - |
? | |
| Protein + H2O | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 44000, gel filtration, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HtrA | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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