Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 43% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
dithiothreitol | 94% inhibition at 10 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
E64 | 31% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
EDTA | 8% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
leupeptin | 96% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
MgCl2 | 21% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
N-alpha-p-tosyl-L-lysine chloromethyl ketone | 12% inhibition at 0.1 mM, with t-butyloxycarbonylc-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
N-ethylmaleimide | 25% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
N-tosyl-L-phenylalanine chloromethyl ketone | 13% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
NaN3 | 34% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus | |
ZnSO4 | 59% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
31000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
41000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
42000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
55000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
62000 | - |
1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, 14880fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
microsomal glutathione S-transferase 1 + H2O | hepsin seems to activate through microsomal glutathione S-transferase 1 dimer formation | Rattus norvegicus | active microsomal glutathione S-transferase | - |
? | |
Suc-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O | 2% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O | 12% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O | 100% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumaryl-7-amide + H2O | 58% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Glu-Ala-Arg-4-methylcoumaryl-7-amide + H2O | 41% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O | 8% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O | 25% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumaryl-7-amide + H2O | 19% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O | 13% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | 20% active with the purifed enzyme | Rattus norvegicus | ? | - |
? | |
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O | 9% active with the purifed enzyme | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
type II transmembrane serine protease | - |
Rattus norvegicus |