F137A |
the mutant shows 14% of the wild type activity |
Haemophilus influenzae |
F144A |
the mutation results in a 42% decrease in activity compared with the wild type enzyme |
Haemophilus influenzae |
F160A |
the mutant decreases peptidase activity by 54% compared to wild type enzyme |
Haemophilus influenzae |
F68A |
the substitution has no effect on activity (98% activity compared to the wild type enzyme) |
Haemophilus influenzae |
F76A |
the mutation results in a 95% decrease in activity compared with the wild type enzyme |
Haemophilus influenzae |
F84A |
the mutant shows 5% of the wild type activity |
Haemophilus influenzae |
L136A |
the mutant shows 22% of the wild type activity |
Haemophilus influenzae |
M164A |
the mutant decreases peptidase activity by 40% compared to wild type enzyme |
Haemophilus influenzae |
W72A |
the mutant is not expressed and activity cannot be assessed |
Haemophilus influenzae |
W72A/F76A/F144A |
the three alanine substitutions result in a 2.5fold increase in activity compared to wild type enzyme |
Haemophilus influenzae |
W72V/F76V/F144V |
the three valine substitutions result in a 2fold increase in activity compared to wild type enzyme |
Haemophilus influenzae |