Cloned (Comment) | Organism |
---|---|
expression of His-tagged GlpG in Escherichia coli strain C43(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged GlpG, hanging drop vapour diffusion method, 5 mg/ml protein in 20 mM HEPES, pH 7.5, 90 mM NaCl, 10% glycerol, and lauryl dimethylamine oxide, mixed with a reservoir solution containing 30% w/v PEG 400, 200 mM CaCl2, and 100 mM MES, pH 6.5, X-ray diffraction structure determination and analysis at 2.25-2.3 A resolution | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme contains 6 transmembrane helices and an N-terminal cytoplasmic part | Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09391 | gene glpG | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged GlpG from membranes of strain C43(DE3) by nickel affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains | structure-function relationship, catalytic residues are Ser201 of transmembrane helix 4 and His254 of transmembrane helix 6 | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling, structure-function relationship, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GlpG | - |
Escherichia coli |
rhomboid serine protease | - |
Escherichia coli |