Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.105 extracted from

  • Wang, Y.; Zhang, Y.; Ha, Y.
    Crystal structure of a rhomboid family intramembrane protease (2006), Nature, 444, 179-180.
    View publication on PubMed
Search for more literature for 3.4.21.105

Crystallization (Commentary)

Crystallization (Comment) Organism
purified GlpG core domain, hanging drop vapour diffusion method, room temperature, 5 mg/ml membrane protein in 10 mM Tris-HCl, pH 7.6, and 20 mM nonylglucoside, over a reservoir solution of 3 M NaCl and 100 mM Bis-Tris propane, pH 7.0, cryoprotection by 25% glycerol, 1 month, X-ray diffraction structure determination and analysis at 2.1 A resolution Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein with six transmembrane segments, membrane topology of a rhomboid protease and its substrate, overview Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli P09391 gene glpG
-

Reaction

Reaction Comment Organism Reaction ID
cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains the active site structure, with a Ser 201 and His 254 catalytic dyad, is accessible by substrate through a large V-shaped opening that faces laterally towards the lipid, but is blocked by a half-submerged loop structure, catalytically involved residues and water molecules, catalytic mechanism, overview Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleave the transmembrane domain of other membrane proteins, membrane topology of a rhomboid protease and its substrate, overview Escherichia coli ?
-
 ?
protein MIC2 + H2O cleavage at an Ala-Gly bond Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
GlpG
-
 Escherichia coli
More the enzyme belongs to the rhomboid intramembrane protease family Escherichia coli