General Stability | Organism |
---|---|
enzyme is stable during at least 10 freeze-thaw cycles | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-2-Macroglobulin | inhibition of recombinant, not native, enzyme at large molar excess | Homo sapiens | |
anti-thrombin III | recombinant enzyme, inhibition only in presence of heparin | Homo sapiens | |
C1 inhibitor | recombinant enzyme, at pH 7.4, inhibitor from human, the protease-serpin complex in highly unstable at alkaline pH | Homo sapiens | |
additional information | no inhibition of recombinant enzyme by thrombin inhibitor Z-D-Phe-Pro-methoxypropylboroglycinepinanediol ester, i.e. boroMpg, and hirudin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM | Homo sapiens | |
NaCl | 140 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | Homo sapiens | - |
2 fragments of complement component C2 | formation of the C3 convertase C4b2a | ? | |
complement component C4 + H2O | Homo sapiens | - |
2 fragments of complement C4 | formation of the C3 convertase C4b2a | ? | |
additional information | Homo sapiens | enzyme is complexed with the mannose-binding protein, a recognition molecule for the complement pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of recombinant enzyme with substrates Phe-Gly-Arg-7-amido-4-methylcoumarin and Phe-Ser-Arg-7-amido-4-methylcoumarin | Homo sapiens |
Storage Stability | Organism |
---|---|
enzyme activity is decreased due to aging, loss of 90% activity after 48-120 h | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | - |
Homo sapiens | 2 fragments of complement component C2 | formation of the C3 convertase C4b2a | ? | |
complement component C2 + H2O | human substrate | Homo sapiens | 2 fragments of complement component C2 | - |
? | |
complement component C4 + H2O | - |
Homo sapiens | 2 fragments of complement C4 | formation of the C3 convertase C4b2a | ? | |
complement component C4 + H2O | human substrate | Homo sapiens | 2 fragments of complement C4 | - |
? | |
additional information | enzyme is complexed with the mannose-binding protein, a recognition molecule for the complement pathway | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, fluorescent amide compounds are poor substrates, overview | Homo sapiens | ? | - |
? | |
Phe-Gly-Arg-7-amido-4-methylcoumarin + H2O | recombinant enzyme, low activity | Homo sapiens | Phe-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | recombinant enzyme, low activity | Homo sapiens | Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MASP-2 | - |
Homo sapiens |
MBP-associated serine protease 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Homo sapiens |