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Literature summary for 3.4.21.104 extracted from

  • Presanis, J.S.; Hajela, K.; Ambrus, G.; Gal, P.; Sim, R.B.
    Differential substrate and inhibitor profiles for human MASP-1 and MASP-2 (2004), Mol. Immunol., 40, 921-929.
    View publication on PubMed

General Stability

General Stability Organism
enzyme is stable during at least 10 freeze-thaw cycles Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha-2-Macroglobulin inhibition of recombinant, not native, enzyme at large molar excess Homo sapiens
anti-thrombin III recombinant enzyme, inhibition only in presence of heparin Homo sapiens
C1 inhibitor recombinant enzyme, at pH 7.4, inhibitor from human, the protease-serpin complex in highly unstable at alkaline pH Homo sapiens
additional information no inhibition of recombinant enzyme by thrombin inhibitor Z-D-Phe-Pro-methoxypropylboroglycinepinanediol ester, i.e. boroMpg, and hirudin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 5 mM Homo sapiens
NaCl 140 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C2 + H2O Homo sapiens
-
2 fragments of complement component C2 formation of the C3 convertase C4b2a ?
complement component C4 + H2O Homo sapiens
-
2 fragments of complement C4 formation of the C3 convertase C4b2a ?
additional information Homo sapiens enzyme is complexed with the mannose-binding protein, a recognition molecule for the complement pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of recombinant enzyme with substrates Phe-Gly-Arg-7-amido-4-methylcoumarin and Phe-Ser-Arg-7-amido-4-methylcoumarin Homo sapiens

Storage Stability

Storage Stability Organism
enzyme activity is decreased due to aging, loss of 90% activity after 48-120 h Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O
-
Homo sapiens 2 fragments of complement component C2 formation of the C3 convertase C4b2a ?
complement component C2 + H2O human substrate Homo sapiens 2 fragments of complement component C2
-
?
complement component C4 + H2O
-
Homo sapiens 2 fragments of complement C4 formation of the C3 convertase C4b2a ?
complement component C4 + H2O human substrate Homo sapiens 2 fragments of complement C4
-
?
additional information enzyme is complexed with the mannose-binding protein, a recognition molecule for the complement pathway Homo sapiens ?
-
?
additional information substrate specificity, fluorescent amide compounds are poor substrates, overview Homo sapiens ?
-
?
Phe-Gly-Arg-7-amido-4-methylcoumarin + H2O recombinant enzyme, low activity Homo sapiens Phe-Gly-Arg + 7-amino-4-methylcoumarin
-
?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O recombinant enzyme, low activity Homo sapiens Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
MASP-2
-
Homo sapiens
MBP-associated serine protease 2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Homo sapiens