Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.103 extracted from

  • Murakami-Murofushi, K.; Takahashi, T.; Minowa, Y.; Lino, S.; Takeuchi, T.; Kitagaki-Ogawa, H.; Murofushi, H.; Takahshi, K.
    Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum (1990), J. Biol. Chem., 32, 19898-19903.
No PubMed abstract available
Search for more literature for 3.4.21.103

Inhibitors

Inhibitors Comment Organism Structure
Diazoacetyl-D,L-norleucine methyl ester in presence of Cu2+ Physarum polycephalum
additional information not: 1,2-epoxy-3-(4-nitrophenoxy)propane Physarum polycephalum
pepstatin not Physarum polycephalum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Physarum polycephalum 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum
31000
-
 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum
68000
-
 gel filtration Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the heavy chain contains a carbohydrate moiety composed of 7 residues mannose, 5 glucosamine, 1 fucose and 1 glucose Physarum polycephalum

Purification (Commentary)

Purification (Comment) Organism
-
 Physarum polycephalum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Physarum polycephalum Hydrolyzed hemoglobin
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Physarum polycephalum Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
additional information derivatives of basic amino acids and related peptides (proteinase E) Physarum polycephalum ?
-
 ?
additional information not: N-acetyl-N-phenylalanyl-3,5-diiodo-L-tyrosine Physarum polycephalum ?
-
 ?
Oxidized insulin B-chain + H2O major cleavage sites: Gly8-Ser9 (most susceptible), Leu11-Val12, Cysteic acid19-Gly20 and Phe24-Phe25 Physarum polycephalum Hydrolyzed insulin B-chain
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 31000 + 1 * 23000, the two chains are cross-linked by disulfide bond(s), SDS-PAGE under reducing and nonreducing conditions Physarum polycephalum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Physarum polycephalum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 3.0-6.0, 48 h, stable Physarum polycephalum
37
-
 15 min, 30% loss of activity at pH 8.0, 45% loss of activity at pH 9.0 Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7
-
 hemoglobin Physarum polycephalum

pH Range

pH Minimum pH Maximum Comment Organism
1.3 3 pH 1.3: about 25% of activity maximum, pH 3.0: about 35% of activity maximum Physarum polycephalum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6
-
 Physarum polycephalum
8
-
 37°C, 15 min, 30% loss of activity Physarum polycephalum
9
-
 37°C, 15 min, 45% loss of activity Physarum polycephalum