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Literature summary for 3.4.21.102 extracted from

  • Duff, S.M.; Chen, Y.S.; Fabbri, B.J.; Yalamanchili, G.; Hamper, B.C.; Walker, D.M.; Brookfield, F.A.; Boyd, E.A.; Ashton, M.R.; Yarnold, C.J.; CaJacob, C.A.
    The carboxyterminal processing protease of D1 protein: herbicidal activity of novel inhibitors of the recombinant and native spinach enzymes (2007), Pestic. Biochem. Physiol., 88, 1-13.
No PubMed abstract available
Search for more literature for 3.4.21.102

Application

Application Comment Organism
agriculture potential target for the discovery of a general broad-spectrum herbicide Spinacia oleracea

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Arabidopsis thaliana, expressed as His-tag fusion protein in Escherichia coli Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
additional information complex inhibitors belonging to the substance classes rhodanine dimer, ketoheterocycles, CF3 peptides, and imidazoles were studied, application of inhibitors to the leaves of Arabidopsis thaliana causes lesions, over expression of CtpA results in enhanced resistance to inhibitor treatment Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pre-D1 protein + H2O Spinacia oleracea catalyzes the conversion of the nascent pre-D1 (pD1) protein into the active form of D1 by cleaving the 9 C-terminal residues in spinach, required to maintain the function of the photosystem II complex D1 protein + peptide
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 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
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-
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Purification (Commentary)

Purification (Comment) Organism
partially purified from spinach, recombinant protein from Escherichia coli using His-tag Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
F-24 peptide + H2O peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein, N-terminally fluorescence-labeled with a fluorescein isothiocyanate group Spinacia oleracea ?
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 ?
pre-D1 protein + H2O catalyzes the conversion of the nascent pre-D1 (pD1) protein into the active form of D1 by cleaving the 9 C-terminal residues in spinach, required to maintain the function of the photosystem II complex Spinacia oleracea D1 protein + peptide
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 ?
SP-4 peptide + H2O peptide substrate comprising the 16 C-terminal residues of spinach pre-D1 protein with an inserted Lys between position 6 and 7 from the C-terminus, the Lys is fluorescence-labeled with a fluorescein isothiocyanate group at the epsilon-amino function, N-terminally biotin-labeled Spinacia oleracea ?
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 ?

Synonyms

Synonyms Comment Organism
carboxyterminal processing protease of D1 protein
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 Spinacia oleracea
CtpA
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 Spinacia oleracea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
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 IC values in the micromolar range found for the effective inhibitors Spinacia oleracea additional information