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Literature summary for 3.4.21.102 extracted from
- Overexpression and characterization of carboxyl-terminal processing protease for precursor D1 protein: regulation of enzyme-substrate interaction by molecular environments (2001), J. Biol. Chem., 276, 7518-7525.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Spinacia oleracea |
| overexpression in Escherichia coli | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | insensitive to penem; not inhibitory: phenylmethylsulfonyl fluoride, diisopropyl fluorophosphate, chymostatin, penem, pepstatin A, 2-mercaptoethanol, N-ethylmaleimide, EDTA, 1,10-phenanthroline | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
C-terminal 19 amino acids of preD1 protein | pH 7.7, 25°C | Spinacia oleracea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Protein + H2O | Spinacia oleracea | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
| recombinant protein | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| C-terminal 19 amino acids of preD1 protein + H2O | replacement of Ala at the cleaving site by Gly, Val, Pro reduces the rate of proteolysis to 50, 30 and 0% of the control | Spinacia oleracea | pre-peptide of D1 + oligomer of 11 amino acids | - |
? | |
| D1 polypeptide of photosystem II + H2O | - |
Spinacia oleracea | ? | - |
? | |
| Protein + H2O | - |
Spinacia oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | strong dependence on pH-value | Spinacia oleracea |
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Release 2023.1 (January 2023)
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