Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
D348A | production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected | Xanthomonas sp. |
DELTA192 | pro-region mutant enzyme | Xanthomonas sp. |
S169A | production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected | Xanthomonas sp. |
S79A | production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected | Xanthomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme DELTA192 | Xanthomonas sp. | |
0.0111 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme E230A | Xanthomonas sp. | |
0.0188 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme E235A | Xanthomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant enzymes S79A, S169A and D348A | Xanthomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Xanthomonas sp. | ? | - |
? | |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O | - |
Xanthomonas sp. | Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XCP | - |
Xanthomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme E235A | Xanthomonas sp. | |
0.9 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme DELTA192 | Xanthomonas sp. | |
21.8 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme E230A | Xanthomonas sp. | |
34.9 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme E235A | Xanthomonas sp. | |
68.4 | - |
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | pH 3.5, 37°C, mutant enzyme DELTA192 | Xanthomonas sp. |