Literature summary for 3.4.21.101 extracted from

  • Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.
    Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium (1987), Biochim. Biophys. Acta, 923, 463-469.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Pseudomonas sp.
chymostatin weak Pseudomonas sp.
HgCl2 slight Pseudomonas sp.
additional information diisopropyl fluorophosphate; EDTA; iodoacetic acid; methyl 2-diazoacetamidohexanoate; not: pepstatin; p-chloromercuribenzenesulfonic acid; phenylmethanesulfonyl fluoride Pseudomonas sp.
PCMB slight Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
1 * 43000, SDS-PAGE Pseudomonas sp.
42000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. No. 101
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information contains less than 0.05% carbohydrate Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
strain No. 101 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Pseudomonas sp. ?
-
?
casein + H2O
-
Pseudomonas sp. No. 101 ?
-
?
Acid-denatured hemoglobin + H2O
-
Pseudomonas sp. ?
-
?
Acid-denatured hemoglobin + H2O
-
Pseudomonas sp. No. 101 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 4.0-5.5, 15 h, stable Pseudomonas sp.
65
-
15 min, complete loss of activity Pseudomonas sp.
45
-
15 min, stable below Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
acid-denatured hemoglobin, casein Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8
-
15 min, stable below 50°C, complete loss of activity at 60°C Pseudomonas sp.
4 5.5 37°C, 15 h, stable Pseudomonas sp.