Literature summary for 3.4.21.1 extracted from

Spelzini, D.; Farruggia, B.; Pico, G.
Purification of chymotrypsin from pancreas homogenate by adsorption onto non-soluble alginate beads (2011), Process Biochem., 46, 801-805.

No PubMed abstract available

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification from an activated homogenate of bovine pancreas by adsorption onto nonsoluble alginate beads. Under all the experimental conditions, the enzyme has a positive net electrical charge, whereas alginate is negatively charged. After performing steps of washing and desorption, chymotrypsin is purified 9times with an enzyme recovery of 62%. The method allows purification with suitable values from a raw sample like pancreas homogenate without a previous clarification step	Bos taurus

Purification (Comment)

Organism

purification from an activated homogenate of bovine pancreas by adsorption onto nonsoluble alginate beads. Under all the experimental conditions, the enzyme has a positive net electrical charge, whereas alginate is negatively charged. After performing steps of washing and desorption, chymotrypsin is purified 9times with an enzyme recovery of 62%. The method allows purification with suitable values from a raw sample like pancreas homogenate without a previous clarification step

Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

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Release 2023.1 (January 2023)