KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C | Bos taurus | |
0.07 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | pH 7.6, 25°C | Bos taurus | |
0.083 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | supramolecular complexes of alpha-chymotrypsin with hydroxyl-containing alkyl ammonium gemini surfactants, i.e. alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl ammonium dibromides), with a polymethylene spacer of varying length. The interaction of the surfactants with alpha-chymotrypsin leads to changes of different intensity in the structural state of proteins. A correlation is found between the activity of alpha-chymotrypsin and the length of the spacer moiety. The enzyme activity correlates with the change in the substrate concentration in the aqueous phase of the surfactant micellar solution | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00766 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-L-tyrosine-4-nitroanilide + H2O | - |
Bos taurus | N-benzoyl-L-tyrosine + 4-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C | Bos taurus | |
0.15 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | pH 7.6, 25°C | Bos taurus | |
0.5 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C | Bos taurus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C | Bos taurus | |
2.14 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | pH 7.6, 25°C | Bos taurus | |
10 | - |
N-benzoyl-L-tyrosine-4-nitroanilide | presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C | Bos taurus |