Application | Comment | Organism |
---|---|---|
biotechnology | dependence of activity on pH and temperature makes chymotrypsin II a biotechnological alternative for food processing when low temperatures are needed like in fish ripening, fish sauce production, fish protein hydrolysate production and other emerging processes. This enzyme together with other proteases from sardine viscera may aid in the enzymatic treatment of stick-water, in which a reduction in viscosity is required for further processing of effluent | Sardinops caeruleus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, inhibits chymotrypsin II, whereas chymotrypsin I is not inhibited | Sardinops caeruleus | |
EDTA | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, inhibits chymotrypsin II, whereas chymotrypsin I is not inhibited | Sardinops caeruleus | |
additional information | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, is not inhibited by benzamidine and EDTA | Bos taurus | |
N-toluenesulfonyl-L-phenylalanine chloromethyl-ketone | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits (53% residual activity) | Bos taurus | |
N-toluenesulfonyl-L-phenylalanine chloromethylketone | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits chymotrypsin I (56% residual activity) and chymotrypsin II (60% residual activity) | Sardinops caeruleus | |
phenylmethylsulfonyl fluoride | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits chymotrypsin I and chymotrypsin II (1-2% residual activity) | Sardinops caeruleus | |
phenylmethylsulfonyl-fluoride | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits (4% residual activity) | Bos taurus | |
Soybean trypsin inhibitor | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits (17% residual activity) | Bos taurus | |
Soybean trypsin inhibitor | at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits chymotrypsin I and chymotrypsin II (no residual activity) | Sardinops caeruleus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | at 30°C, chymotrypsin II | Sardinops caeruleus | |
0.074 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | at 30°C, chymotrypsin I | Sardinops caeruleus | |
0.09 | - |
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide | at 30°C | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
gel filtration | Sardinops caeruleus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Sardinops caeruleus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chymotrypsin I and II, by ionic exchange chromatography | Sardinops caeruleus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36 | - |
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, on the amide substrate | Bos taurus |
39 | - |
chymotrypsin II, on the amide substrate, at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2 | Sardinops caeruleus |
58 | - |
chymotrypsin I, on the amide substrate, at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2 | Sardinops caeruleus |
84 | - |
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, on the ester substrate | Bos taurus |
109 | - |
chymotrypsin II, on the ester substrate, at 25°C, in 50 mM Tris-HCl buffer and 10 mM CaCl2 | Sardinops caeruleus |
168 | - |
chymotrypsin I, on the ester substrate, at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2 | Sardinops caeruleus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-L-tyrosine ethyl ester + H2O | - |
Bos taurus | ? | - |
? | |
benzoyl-L-tyrosine ethyl ester + H2O | - |
Sardinops caeruleus | benzoyl-L-tyrosine + ethanol | - |
? | |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | - |
Sardinops caeruleus | succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide + H2O | - |
Bos taurus | succinyl-L-Ala-L-Ala-L-Pro-L-Phe + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chymotrypsin | - |
Bos taurus |
chymotrypsin I | - |
Sardinops caeruleus |
chymotrypsin II | - |
Sardinops caeruleus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | activity of chymotrypsin I and II increases with temperature up to an optimum of 50 and 45°C, respectively, when assayed against succinyl-Ala-Ala-Pro-Phe-p-nitroanilide at pH 8.0 | Sardinops caeruleus |
55 | - |
activity increases with temperature up to an optimum of 55°C, when assayed against succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide at pH 8 | Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | chymotrypsin I shows high activity in the range from 20°C to 50°C and chymotrypsin II from 10°C to 45°C | Sardinops caeruleus |
55 | 70 | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
chymotrypsin I is completely inactivated at 55°C and chymotrypsin II at 50°C | Sardinops caeruleus |
40 | - |
is inactivated at 60°C | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide | at 30°C | Bos taurus | |
4.8 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | at 30°C, chymotrypsin II | Sardinops caeruleus | |
18.6 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | at 30°C, chymotrypsin I | Sardinops caeruleus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
at 25°C, with succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide as substrate | Bos taurus |
8 | - |
chymotrypsin I and II, at 25°C, with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as substrate | Sardinops caeruleus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | low activity chymotrypsin I and II at acidic pH (45), considerably activity at pH from 6 to 9 | Bos taurus |
4 | 11 | low activity of chymotrypsin I and II at acidic pH (4-5), considerably activity at pH from 6 to 9 whilst chymotrypsin I shows the highest activity toward the alkaline pH (7-11) | Sardinops caeruleus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
is stable at acid and alkaline pH | Bos taurus |
6 | 11 | chymotrypsin I and chymotrypsin II, are most stable at alkaline pH | Sardinops caeruleus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sardinops caeruleus | chymotrypsin I, electrofocusing | - |
4.3 |
Sardinops caeruleus | chymotrypsin II, electrofocusing | - |
5.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide | at 30°C | Bos taurus |