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Literature summary for 3.4.21.1 extracted from

  • Hong, J.; Xu, D.; Gong, P.; Sun, H.; Dong, L.; Yao, S.
    Covalent binding of alpha-chymotrypsin on the magnetic nanogels covered by amino groups (2007), J. Mol. Catal. B, 45, 84-90.
No PubMed abstract available
Search for more literature for 3.4.21.1

Organism

Organism UniProt Comment Textmining
Bos taurus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
25°C, free enzyme in 80 mM Tris-HCl at pH 7.8, 22 days, complete loss of activity Bos taurus
25°C, nanogel-immobilized enzyme, 36 days, about 10% loss of activity Bos taurus
4°C, free enzyme in 80 mM Tris-HCl at pH 7.8, 36 days, 16.5% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosine ethyl ester + H2O
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 Bos taurus N-benzoyl-L-tyrosine + ethanol
-
 ?

Synonyms

Synonyms Comment Organism
alpha-chymotrypsin
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75 85 almost no activity is retained when the incubation temperature is above 75°C, the activity of the nanogel-bound alpha-CT still has a residual activity of 88.7% at 85°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 when immobilized on on magnetic nanogels Bos taurus