Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 0.25 mg/ml EDTA or 2 mg/ml benzamidine | Sardinops sagax | |
N-toluenesulfonyl-L-phenylalanine-chloromethyl ketone | 56% residual activity at 1 mg/ml | Sardinops sagax | |
Phenylmethylsulfonylfluoride | 1% residual activity at 1.4 mg/ml | Sardinops sagax | |
Soybean trypsin inhibitor | 6% residual activity at 0.5 mg/ml | Sardinops sagax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide | in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C | Sardinops sagax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
SDS-PAGE | Sardinops sagax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sardinops sagax | - |
Sardinops sagax caerulea, Monterey sardine | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography | Sardinops sagax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
visceral muscle | - |
Sardinops sagax | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
crude extract, at 30°C | Sardinops sagax |
43 | - |
after 7.1fold purification, at 30°C | Sardinops sagax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-L-tyrosine ethyl ester + H2O | - |
Sardinops sagax | N-benzoyl-L-tyrosine + ethanol | - |
? | |
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide + H2O | - |
Sardinops sagax | N-succinyl-L-Ala-Ala-Pro + p-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sardinops sagax |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | activity quickly decreases at temperatures above the optimum, the relative activity of the enzyme at 25°C is 60% of maximum activity, the enzyme is labile at high temperatures (more than 55°C) and stabilized at less than 30°C | Sardinops sagax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.6 | - |
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide | in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C | Sardinops sagax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sardinops sagax |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | retains more than 80% activity over the pH range 5-10 | Sardinops sagax |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | high stability at pH 7-8, unstable below pH 5 | Sardinops sagax |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sardinops sagax | pI about 4.3, isoelectric focusing | - |
4.3 |