Literature summary for 3.4.21.1 extracted from

Celej, M.S.; Dandrea, M.G.; Campana, P.T.; Fidelio, G.D.; Bianconi, M.L.
Superactivity and conformational changes on alpha-chymotrypsin upon interfacial binding to cationic micelles (2004), Biochem. J., 378, 1059-1066.

Search for more literature for 3.4.21.1

Activating Compound

Activating Compound	Comment	Organism	Structure
hexadecyltrimethylammonium bromide	2-60 mM, aqueous micelles of the cationic polymeric surfactant hexadecyltrimethylammonium bromide	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0297	-	p-nitrophenyl acetate	in the presence of 20 mM hexadecyltrimethylammonium bromide, at 25°C	Bos taurus
0.044	-	p-nitrophenyl acetate	at 25°C	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl acetate + H2O	-	Bos taurus	p-nitrophenol + acetate	-	?

Synonyms

Synonyms	Comment	Organism
alpha-chymotrypsin	-	Bos taurus
alpha-CT	-	Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	retaining around 85% of the initial activity after 2 h	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.017	-	p-nitrophenyl acetate	in 20 mM Tris/HCl pH 7.5 at 25°C	Bos taurus
0.028	-	p-nitrophenyl acetate	in the presence of 20 mM hexadecyltrimethylammonium bromide, in 20 mM Tris/HCl pH 7.5 at 25°C	Bos taurus

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Release 2023.1 (January 2023)