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Literature summary for 3.4.21.1 extracted from
- Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas (2005), Appl. Biochem. Microbiol., 41, 139-144.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | treatment of gastroenterological diseases | Silurus glanis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39800 | - |
Sephadex G-75 superfine gel chromatography | Silurus glanis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Silurus glanis | - |
European catfish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration and ion exchange chromatography | Silurus glanis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Silurus glanis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Kh1: 0.25 units with milk-acetate mixture substrate before purification and 1.3 units with milk-acetate mixture substrate after 5.2fold purification at 35°C, Kh2: 0.25 units with milk-acetate mixture substrate before purification and 1.8 units with milk-acetate mixture substrate after 7.2fold purification at 35°C, Kh3: 0.25 units with milk-acetate mixture substrate before purification and 4.1 units with milk-acetate mixture substrate after 16.4fold purification at 35°C (1 unit is defined as the amount of enzyme that clots 5 ml of milk-acetate mixture during 1 min) | Silurus glanis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| milk-acetate mixture + H2O | - |
Silurus glanis | ? | - |
? |  |
| SPPNA + H2O | specific substrate for chymotrypsin | Silurus glanis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chymotrypsin isoform Kh1 | - |
Silurus glanis |
| chymotrypsin isoform Kh2 | - |
Silurus glanis |
| chymotrypsin isoform Kh3 | - |
Silurus glanis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
chymotrypsin isoform Kh2 | Silurus glanis |
| 9.5 | - |
chymotrypsin isoform Kh3 | Silurus glanis |
| 10.5 | - |
chymotrypsin isoform Kh1 | Silurus glanis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | chymotrypsin isoform Kh2 | Silurus glanis |
| 5 | 9 | chymotrypsin isoform Kh1 | Silurus glanis |
| 5 | 7 | chymotrypsin isoform Kh3 | Silurus glanis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Silurus glanis | chymotrypsin isoform Kh1 | - |
4.93 |
| Silurus glanis | chymotrypsin isoform Kh2 | - |
5.23 |
| Silurus glanis | chymotrypsin isoform Kh3 | - |
6.18 |
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Release 2023.1 (January 2023)
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