Literature summary for 3.4.21.1 extracted from

Ghani, U.; Ng, K.K.; Atta ur, R.; Choudhary, M.I.; Ullah, N.; James, M.N.
Crystal structure of gamma-chymotrypsin in complex with 7-hydroxycoumarin (2001), J. Mol. Biol., 314, 519-525.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are grown from 52% ammonium sulfate as precipitant containing 10 mg/ml of gamma-chymotrypsin, 100 mM sodium-cacodylate, pH 7.0 and 2.5% dioxane using the hanging-drop vapor diffusion method, crystals of gamma-chymotrypsin complexed with 7-hydroxycoumarin diffract to 1.8 A	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
7-hydroxycoumarin	0.78 mM, 50% inhibition	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00766	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O	-	Bos taurus	N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline	-	?

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Release 2023.1 (January 2023)