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Literature summary for 3.4.21.1 extracted from
- Stabilization of alpha-chymotrypsin by modification with beta-cyclodextrin derivatives (2002), Biotechnol. Appl. Biochem., 36, 235-239.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | Bos taurus | - |
fragments of casein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0034 | - |
casein proteolysis | Bos taurus |
| 44.6 | - |
hydrolysis of N-alpha-acetyl-L-tyrosine ethyl ester | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Bos taurus | fragments of casein | - |
? | |
| N-alpha-acetyl-L-tyrosine ethyl ester + H2O | - |
Bos taurus | N-alpha-acetyl-L-tyrosine + ethanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-chymotrypsin | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bos taurus |
| 58 | - |
after modification of alpha-chymotrypsin with mono-6-formyl-beta-cyclodextrin and mono-6-succinyl-6-deoxy-beta-cyclodextrin | Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 62 | approx. 50% of maximal activity at 20°C, approx. 20% of maximal activity at 62°C | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability of alpha-chymotrypsin is enhanced by about 4-6°C after modification with mono-6-formyl-beta-cyclodextrin and mono-6-succinyl-6-deoxy-beta-cyclodextrin | Bos taurus |
| 35 | 55 | approx. 50% loss of activity after 10 min at 50°C, complete loss of activity after 10 min at 55°C | Bos taurus |
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