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Literature summary for 3.4.21.1 extracted from
- Purification, characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta (1995), Insect Biochem. Mol. Biol., 25, 765-774.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Manduca sexta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chymostatin | - |
Manduca sexta |  |
| DFP | - |
Manduca sexta | |
| PMSF | - |
Manduca sexta | |
| TPCK | - |
Manduca sexta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23600 | - |
gel filtration | Manduca sexta |
| 24000 | - |
x * 24000, SDS-PAGE | Manduca sexta |
| 24852 | - |
x * 24852, calculation from nucleotide sequence | Manduca sexta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manduca sexta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Manduca sexta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | - |
Manduca sexta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specific hydrolysis of peptide bonds on the carboxyl side of Tyr, Phe, Trp, His, Leu Thr and Gly | Manduca sexta | ? | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide + H2O | - |
Manduca sexta | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Manduca sexta |
| ? | x * 24852, calculation from nucleotide sequence | Manduca sexta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | 11 | - |
Manduca sexta |
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