Cloned (Comment) | Organism |
---|---|
cloned and inserted into a pET43.1a vector via SmaI and EcoRI sites and expressed in Rosetta (DE3) cells as a Nus-His-tag fusion enzyme (NH-zgammaGH) | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
5-formyltetrahydrofolate triglutamate | Vmax: 87.6 nmol/min/microg | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant NH-zgammaGH and zgammaGH appear on SDS-PAGE in the ranges of their estimated size of 92 and 33 kDa, respectively | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Danio rerio | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | ? | - |
? | |
additional information | Danio rerio AB | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6NY42 | AB strain | - |
Danio rerio AB | Q6NY42 | AB strain | - |
Purification (Comment) | Organism |
---|---|
after induction with isopropyl thiogalactoside, the enzyme is purified with a Ni-Sepharose column | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyltetrahydrofolate triglutamate + H2O | - |
Danio rerio | ? | - |
? | |
5-formyltetrahydrofolate triglutamate + H2O | - |
Danio rerio AB | ? | - |
? | |
additional information | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | Danio rerio | ? | - |
? | |
additional information | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | Danio rerio AB | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | zgammaGH. NH-zgammaGH is a tetramer. It seems that the presence of the N-terminal Nus and His-tag interferes with the quaternary structure of zgammaGH | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl hydrolase | - |
Danio rerio |
gammaGH | - |
Danio rerio |
zgammaGH | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.2 | - |
5-formyltetrahydrofolate triglutamate | Vmax: 87.6 nmol/min/microg | Danio rerio |