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Literature summary for 3.4.19.9 extracted from

  • Chunhachart, O.; Itoh, T.; Sukchotiratana, M.; Tanimoto, H.; Tahara, Y.
    Characterization of gamma-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao (2006), Biosci. Biotechnol. Biochem., 70, 2779-2782.
    View publication on PubMed
Search for more literature for 3.4.19.9

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 40% inhibition at 5 mM Bacillus sp. (in: Bacteria)
additional information no inhibition by EDTA, PMSF, or iodoacetic acid at 5 mM Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Co2+, Mg2+, Mn2+, and Ni2+ at 5 mM have little effect on enzyme activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 NBRC16449, CMU29, isolated from Thai Thua-nao, a natto-like fermented soybean food
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture broth Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(poly-gamma-L-glutamate)n + H2O
-
 Bacillus sp. (in: Bacteria) oligo-gamma-L-glutamate
-
 ?
di-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
additional information the enzyme hydrolyzes synthetically hydrolyzed oligo-gamma-L-glutamates, but not oligo-gamma-D-glutamates, and degrades polyglutamic acid to a hydrolyzed product of about 20 kDa with a ratio of D- and L-glutamic acids of 70:30, thus the enzyme cleaves the bond between two L-glutamic acid residues, overview Bacillus sp. (in: Bacteria) ?
-
 ?
oligo-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
tetra-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
tri-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, pH 8.0, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus sp. (in: Bacteria)