Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 40% inhibition at 5 mM | Bacillus sp. (in: Bacteria) | |
additional information | no inhibition by EDTA, PMSF, or iodoacetic acid at 5 mM | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Co2+, Mg2+, Mn2+, and Ni2+ at 5 mM have little effect on enzyme activity | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
NBRC16449, CMU29, isolated from Thai Thua-nao, a natto-like fermented soybean food | - |
Purification (Comment) | Organism |
---|---|
native enzyme from culture broth | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(poly-gamma-L-glutamate)n + H2O | - |
Bacillus sp. (in: Bacteria) | oligo-gamma-L-glutamate | - |
? | |
di-gamma-L-glutamate + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamate | - |
? | |
additional information | the enzyme hydrolyzes synthetically hydrolyzed oligo-gamma-L-glutamates, but not oligo-gamma-D-glutamates, and degrades polyglutamic acid to a hydrolyzed product of about 20 kDa with a ratio of D- and L-glutamic acids of 70:30, thus the enzyme cleaves the bond between two L-glutamic acid residues, overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
oligo-gamma-L-glutamate + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamate | - |
? | |
tetra-gamma-L-glutamate + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamate | - |
? | |
tri-gamma-L-glutamate + H2O | - |
Bacillus sp. (in: Bacteria) | L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, pH 8.0, stable | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |