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Literature summary for 3.4.19.9 extracted from
- Characterization of human gamma-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer (2006), Biochim. Biophys. Acta, 1764, 1479-1486.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminally His-tagged enzyme in Hi5 insect cells | Rattus norvegicus |
| expression the N-terminally His-tagged and non-tagged glycosylated enzyme in Hi5 insect cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H220N | site-directed mutagenesis, inactive mutant | Homo sapiens |
| additional information | the enzyme forms very stable dimeric complexes with the glycosylated human enzyme, overview | Rattus norvegicus |
| additional information | the recombinant glycosylated enzyme forms very stable dimeric complexes with the recombinant His-tagged rat enzyme, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Homo sapiens | 5622 | - |
| intracellular | - |
Rattus norvegicus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35938 | - |
2 * 35938, recombinant His-tagged enzyme, dynamic light scattering, analytical ultracentrifugation, and sedimentation velocity measurement, nondissociating homodimer, homodimer formation is required for folding into the active conformation, overview | Homo sapiens |
| 71880 | - |
recombinant His-tagged enzyme, dynamic light scattering, analytical ultracentrifugation, and sedimentation velocity measurement | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | key enzyme in the metabolism of folic acid and the pharmacology of many antifolate drugs | ? | - |
? |  |
| additional information | Rattus norvegicus | key enzyme in the metabolism of folic acid and the pharmacology of many antifolate drugs | ? | - |
? | |
| polyglutamyl-folate + H2O | Homo sapiens | - |
monoglutamyl folate + glutamate | - |
? | |
| polyglutamyl-folate + H2O | Rattus norvegicus | - |
monoglutamyl folate + glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | Q62867 | gene Ggh | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Hi5 cells to homogeneity | Rattus norvegicus |
| recombinant N-terminally His-tagged enzyme from Hi5 insect cells, and non-tagged glycosylated enzyme from Hi5 insect cells by anion exchange chromatography | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| tetrahydropteroyl-(gamma-glutamyl)n + (n-1) H2O = 5,6,7,8-tetrahydrofolate + (n-1) L-glutamate | Tyr36 is catalytically essential | Homo sapiens | |
| tetrahydropteroyl-(gamma-glutamyl)n + (n-1) H2O = 5,6,7,8-tetrahydrofolate + (n-1) L-glutamate | Tyr36 is catalytically essential | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | key enzyme in the metabolism of folic acid and the pharmacology of many antifolate drugs | Homo sapiens | ? | - |
? | |
| additional information | key enzyme in the metabolism of folic acid and the pharmacology of many antifolate drugs | Rattus norvegicus | ? | - |
? | |
| polyglutamyl-folate + H2O | - |
Homo sapiens | monoglutamyl folate + glutamate | - |
? | |
| polyglutamyl-folate + H2O | - |
Rattus norvegicus | monoglutamyl folate + glutamate | - |
? | |
| polyglutamyl-folate + H2O | removal of the poly-gamma-glutamate chains | Homo sapiens | monoglutamyl folate + glutamate | - |
? | |
| polyglutamyl-folate + H2O | removal of the poly-gamma-glutamate chains | Rattus norvegicus | monoglutamyl folate + glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35938, recombinant His-tagged enzyme, dynamic light scattering, analytical ultracentrifugation, and sedimentation velocity measurement, nondissociating homodimer, homodimer formation is required for folding into the active conformation, overview | Homo sapiens |
| dimer | nondissociating homodimer, homodimer formation is required for folding into the active conformation, overview | Rattus norvegicus |
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