Cloned (Comment) | Organism |
---|---|
expression with a baculovirus/Sf9 insect cell expression system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | inhibition of wild-type enzyme and mutant enzymes C19A, C124A and C290A | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
baculovirus-expressed enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HL-60 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methotrexate diglutamate + H2O | poor substrate | Homo sapiens | ? | - |
? | |
methotrexate hexaglutamate + H2O | - |
Homo sapiens | methotrexate pentaglutamate + methotrexate tetraglutamate + methotrexate triglutamate + methotrexate diglutamate + methotrexate monoglutamate | - |
? | |
methotrexate pentaglutamate + H2O | - |
Homo sapiens | methotrexate tetraglutamate + methotrexate triglutamate + methotrexate diglutamate + methotrexate monoglutamate + L-glutamate | - |
? | |
methotrexate tetraglutamate + H2O | - |
Homo sapiens | ? | - |
? | |
methotrexate triglutamate + H2O | - |
Homo sapiens | methotrexate monoglutamate + methotrexate diglutamate + glutamate | - |
? | |
additional information | the maximal velocity decreases in the order methothrexate hexaglutamate, methotrexate tetraglutamate, methotrexate pentaglutamate, methotrexate triglutamate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Homo sapiens |