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Literature summary for 3.4.19.9 extracted from

  • Muro, T.; Nagamori, Y.; Okada, S.; Tominaga, Y.
    Some properties and action of poly(glutamic acid) hydrolase II from Micromonospora melanosporea IFO 12515 (1990), Agric. Biol. Chem., 54, 1065-1067.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Micromonospora melanosporea
EDTA
-
Micromonospora melanosporea
additional information no inhibition by diisopropylfluorophosphate Micromonospora melanosporea
Ni2+
-
Micromonospora melanosporea
o-phenanthroline
-
Micromonospora melanosporea
PCMB
-
Micromonospora melanosporea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration Micromonospora melanosporea

Organism

Organism UniProt Comment Textmining
Micromonospora melanosporea
-
IFO 12515, 2 poly(glutamic acid) hydrolases: PGH-I and PGH-II
-

Purification (Commentary)

Purification (Comment) Organism
PGH-II Micromonospora melanosporea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly-Glu + H2O
-
Micromonospora melanosporea ? Glu-Glu + oligomers of Glu ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Micromonospora melanosporea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 4.0-10.0, 60 min, stable Micromonospora melanosporea
50
-
below, pH 9.0, 15 min, stable Micromonospora melanosporea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Micromonospora melanosporea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 40°C, 60 min, stable Micromonospora melanosporea