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Literature summary for 3.4.19.7 extracted from

  • Suda, H.; Yamamoto, K.; Aoyagi, T.; Umezawa, H.
    Purification and properties of N-formylmethionine aminopeptidase from rat liver (1980), Biochim. Biophys. Acta, 616, 60-67.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Rattus norvegicus
Cu2+ moderate Rattus norvegicus
Fe2+ moderate Rattus norvegicus
heavy metal ions
-
Rattus norvegicus
Hg2+
-
Rattus norvegicus
additional information not: EDTA Rattus norvegicus
NEM weak Rattus norvegicus
phenylmethanesulfonyl fluoride weak Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
N-Formyl-Met-Leu
-
Rattus norvegicus
0.2
-
N-Formyl-Met 2-naphthylamide
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- activates Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
290000
-
rat, gel filtration Rattus norvegicus
72000
-
4 * 72000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information highly specific for N-formyl-Met peptides, will not cleave methionyl peptides or N-formyl derivatives of amino acids other than methionine Rattus norvegicus ?
-
?
N-Formyl-Met-Leu + H2O
-
Rattus norvegicus N-Formyl-Met + Leu
-
?
N-Formyl-Met 2-naphthylamide + H2O
-
Rattus norvegicus N-Formyl-Met + 2-naphthylamine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 72000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
230
-
N-Formyl-Met 2-naphthylamide
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 6: about 40% of activity maximum, 10: about 50% of activity maximum Rattus norvegicus