Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Rattus norvegicus | |
Cu2+ | moderate | Rattus norvegicus | |
Fe2+ | moderate | Rattus norvegicus | |
heavy metal ions | - |
Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
additional information | not: EDTA | Rattus norvegicus | |
NEM | weak | Rattus norvegicus | |
phenylmethanesulfonyl fluoride | weak | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
0.2 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | activates | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
4 * 72000, SDS-PAGE | Rattus norvegicus |
290000 | - |
rat, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | highly specific for N-formyl-Met peptides, will not cleave methionyl peptides or N-formyl derivatives of amino acids other than methionine | Rattus norvegicus | ? | - |
? | |
N-Formyl-Met 2-naphthylamide + H2O | - |
Rattus norvegicus | N-Formyl-Met + 2-naphthylamine | - |
? | |
N-Formyl-Met-Leu + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 72000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
230 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 6: about 40% of activity maximum, 10: about 50% of activity maximum | Rattus norvegicus |