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Literature summary for 3.4.19.7 extracted from
- Purification and properties of N-formylmethionine aminopeptidase from rat liver (1980), Biochim. Biophys. Acta, 616, 60-67.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Rattus norvegicus |  |
| Cu2+ | moderate | Rattus norvegicus | |
| Fe2+ | moderate | Rattus norvegicus | |
| heavy metal ions | - |
Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| additional information | not: EDTA | Rattus norvegicus | |
| NEM | weak | Rattus norvegicus | |
| phenylmethanesulfonyl fluoride | weak | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
| 0.2 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | activates | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, SDS-PAGE | Rattus norvegicus |
| 290000 | - |
rat, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | highly specific for N-formyl-Met peptides, will not cleave methionyl peptides or N-formyl derivatives of amino acids other than methionine | Rattus norvegicus | ? | - |
? | |
| N-Formyl-Met 2-naphthylamide + H2O | - |
Rattus norvegicus | N-Formyl-Met + 2-naphthylamine | - |
? | |
| N-Formyl-Met-Leu + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 72000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 230 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 6: about 40% of activity maximum, 10: about 50% of activity maximum | Rattus norvegicus |
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