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Literature summary for 3.4.19.5 extracted from
- Structural and kinetic characterization of guinea pig L-asparaginase type III (2014), Biochemistry, 53, 2318-2328.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | isoform ASRGL1 is not the asparaginase responsible for the antitumor effects elicited by treatment with guinea pig serum | Cavia porcellus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cavia porcellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| uncleaved (inactive) as well as the cleaved (active), aspartate-bound structures. Cleavage does not greatly alter the overall structure of the enzyme with the exception of the conformational change in the conserved HGG loop that coincides with cleavage | Cavia porcellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
beta-Asp-Phe | pH 7.5, 37°C | Cavia porcellus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
beta-subunit, SDS-PAGE | Cavia porcellus |
| 20000 | - |
alpha-subunit, SDS-PAGE | Cavia porcellus |
| 35000 | - |
inactive precursor, SDS-PAGE | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | inactive precursor undergoes a self-activation process, converting it from an uncleaved precursor of 35000 Da into alpha- and beta-subunits of 20000 and 15000 Da, respectively. Glycine stimulates cleavage in a dose-dependent manner | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-Asp-Phe + H2O | - |
Cavia porcellus | L-Asp + L-Phe | - |
? | |
| additional information | enzyme additionally acts as an asparaginase, EC 3.5.1.1 | Cavia porcellus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASNase3 | - |
Cavia porcellus |
| ASRGL1 | - |
Cavia porcellus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.98 | - |
beta-Asp-Phe | pH 7.5, 37°C | Cavia porcellus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.83 | - |
beta-Asp-Phe | pH 7.5, 37°C | Cavia porcellus | |
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