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Literature summary for 3.4.19.3 extracted from
- One step purification and characterization of the pyrrolidone carboxyl peptidase of Streptococcus pyogenes over-expressed in Escherichia coli (1992), FEBS Lett., 308, 70-74.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Streptococcus pyogenes |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, 67% of the activity is recovered after one cycle, 70% loss of activity after 5 cycles | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Pyrrolidone | competitive | Streptococcus pyogenes |  |
| Co2+ | - |
Streptococcus pyogenes | |
| Hg2+ | - |
Streptococcus pyogenes | |
| iodoacetamide | - |
Streptococcus pyogenes | |
| iodoacetate | - |
Streptococcus pyogenes | |
| Zn2+ | - |
Streptococcus pyogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.79 | - |
Pyroglutamyl-beta-naphthylamide | - |
Streptococcus pyogenes | |
| 20 | - |
pyroglutamyl-p-nitroanilide | - |
Streptococcus pyogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
4 * 23500, SDS-PAGE | Streptococcus pyogenes |
| 85000 | - |
nondenaturing PAGE | Streptococcus pyogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus pyogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.8 | - |
- |
Streptococcus pyogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, almost 60% of the initial activity is recovered after 1 month | Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyroglutamyl-beta-naphthylamide + H2O | - |
Streptococcus pyogenes | pyroglutamate + beta-naphthylamine | - |
? | |
| pyroglutamyl-p-nitroanilide + H2O | - |
Streptococcus pyogenes | pyroglutamate + p-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 23500, SDS-PAGE | Streptococcus pyogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month | Streptococcus pyogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptococcus pyogenes |
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