Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptococcus pyogenes |
General Stability | Organism |
---|---|
freezing and thawing, 67% of the activity is recovered after one cycle, 70% loss of activity after 5 cycles | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Pyrrolidone | competitive | Streptococcus pyogenes | |
Co2+ | - |
Streptococcus pyogenes | |
Hg2+ | - |
Streptococcus pyogenes | |
iodoacetamide | - |
Streptococcus pyogenes | |
iodoacetate | - |
Streptococcus pyogenes | |
Zn2+ | - |
Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.79 | - |
Pyroglutamyl-beta-naphthylamide | - |
Streptococcus pyogenes | |
20 | - |
pyroglutamyl-p-nitroanilide | - |
Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
4 * 23500, SDS-PAGE | Streptococcus pyogenes |
85000 | - |
nondenaturing PAGE | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.8 | - |
- |
Streptococcus pyogenes |
Storage Stability | Organism |
---|---|
4°C, almost 60% of the initial activity is recovered after 1 month | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyroglutamyl-beta-naphthylamide + H2O | - |
Streptococcus pyogenes | pyroglutamate + beta-naphthylamine | - |
? | |
pyroglutamyl-p-nitroanilide + H2O | - |
Streptococcus pyogenes | pyroglutamate + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 23500, SDS-PAGE | Streptococcus pyogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus pyogenes |