Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.3 extracted from

  • Le Saux, O.; Gonzales, T.; Robert-Baudouy, J.
    Mutational analysis of the active site of Pseudomonas fluorescens pyrrolidone carboxyl peptidase (1996), J. Bacteriol., 178, 3308-3313.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, wild-type and mutant enzymes Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
C144A inactive enzyme Pseudomonas fluorescens
D89A no activity Pseudomonas fluorescens
D89N 35% of activity of the wild-type enzyme Pseudomonas fluorescens
E10Q enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to those of wild-type enzyme Pseudomonas fluorescens
E22Q enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to wild-type enzyme Pseudomonas fluorescens
H166S inactive enzyme Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas fluorescens
0.05
-
Pyroglutamyl-beta-naphthylamide mutant enzyme D89N Pseudomonas fluorescens
0.21
-
Pyroglutamyl-beta-naphthylamide wild-type enzyme Pseudomonas fluorescens
0.22
-
Pyroglutamyl-beta-naphthylamide mutant enzyme E22Q Pseudomonas fluorescens
0.24
-
Pyroglutamyl-beta-naphthylamide mutant enzyme E10Q Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyroglutamyl-Ala + H2O
-
Pseudomonas fluorescens pyroglutamate + Ala
-
?
pyroglutamyl-beta-naphthylamide + H2O
-
Pseudomonas fluorescens pyroglutamate + beta-naphthylamine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.4
-
Pyroglutamyl-beta-naphthylamide mutant enzyme D89N Pseudomonas fluorescens
28
-
Pyroglutamyl-beta-naphthylamide mutant enzyme E10Q Pseudomonas fluorescens
31.4
-
Pyroglutamyl-beta-naphthylamide wild-type enzyme Pseudomonas fluorescens
35.1
-
Pyroglutamyl-beta-naphthylamide mutant enzyme E22Q Pseudomonas fluorescens