Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and mutant enzymes | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
C144A | inactive enzyme | Pseudomonas fluorescens |
D89A | no activity | Pseudomonas fluorescens |
D89N | 35% of activity of the wild-type enzyme | Pseudomonas fluorescens |
E10Q | enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to those of wild-type enzyme | Pseudomonas fluorescens |
E22Q | enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to wild-type enzyme | Pseudomonas fluorescens |
H166S | inactive enzyme | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas fluorescens | |
0.05 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme D89N | Pseudomonas fluorescens | |
0.21 | - |
Pyroglutamyl-beta-naphthylamide | wild-type enzyme | Pseudomonas fluorescens | |
0.22 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme E22Q | Pseudomonas fluorescens | |
0.24 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme E10Q | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyroglutamyl-Ala + H2O | - |
Pseudomonas fluorescens | pyroglutamate + Ala | - |
? | |
pyroglutamyl-beta-naphthylamide + H2O | - |
Pseudomonas fluorescens | pyroglutamate + beta-naphthylamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme D89N | Pseudomonas fluorescens | |
28 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme E10Q | Pseudomonas fluorescens | |
31.4 | - |
Pyroglutamyl-beta-naphthylamide | wild-type enzyme | Pseudomonas fluorescens | |
35.1 | - |
Pyroglutamyl-beta-naphthylamide | mutant enzyme E22Q | Pseudomonas fluorescens |