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Literature summary for 3.4.19.3 extracted from

  • Tsuru, D.; Sakabe, K.; Yoshimoto, T.; Fujiwara, K.
    Pyroglutamyl peptidase from chicken liver: purification and some properties (1982), J. Pharmacobio-Dyn., 5, 859-868.
    View publication on PubMed
Search for more literature for 3.4.19.3

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline weak activation Gallus gallus
DFP weak activation Gallus gallus
EDTA weak activation Gallus gallus
PMSF weak activation Gallus gallus

General Stability

General Stability Organism
2-mercaptoethanol and EDTA stabilize Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Gallus gallus
Hg2+
-
 Gallus gallus
iodoacetate
-
 Gallus gallus
L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane i.e. E-64, partial Gallus gallus
Ni2+
-
 Gallus gallus
PCMB
-
 Gallus gallus
peptidic calpain inhibitor i.e. EP-475, partial Gallus gallus
pyroglutamyl-D-Ala
-
 Gallus gallus
pyroglutamyl-Pro
-
 Gallus gallus
Sodium tetrathionate
-
 Gallus gallus
Zn2+
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 pyroglutamate-4-methylcoumarin 7-amide
-
 Gallus gallus
0.7
-
 pyroglutamyl-2-naphthylamide
-
 Gallus gallus
0.73
-
 pyroglutamyl-p-nitroanilide
-
 Gallus gallus
0.89
-
 L-pyroglutamyl-L-Ala
-
 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.45
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, rapid inactivation in presence of EDTA and 2-mercaptoethanol Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin potentiator + H2O
-
 Gallus gallus pyroglutamate + ?
-
 ?
L-pyroglutamyl-L-Ala + H2O
-
 Gallus gallus L-pyroglutamate + L-Ala
-
 ?
litorin + H2O frog litorin Gallus gallus pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2
-
 ?
luteinizing-hormone-releasing hormone + H2O
-
 Gallus gallus ?
-
 ?
additional information the enzyme is inactive towards uperolein and piperidone carbonyl His-Pro-NH2 Gallus gallus ?
-
 ?
N-(4-methyl-2-oxo-2H-chromen-7-yl)-5-oxo-L-prolinamide + H2O
-
 Gallus gallus 5-oxo-L-proline + 7-amino-4-methylcoumarin
-
 ?
neurotensin + H2O
-
 Gallus gallus pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
 ?
physalaemin + H2O
-
 Gallus gallus pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2
-
 ?
pyroglutamyl-beta-naphthylamide + H2O
-
 Gallus gallus pyroglutamate + beta-naphthylamine
-
 ?
pyroglutamyl-D-Ala + H2O slight activity Gallus gallus pyroglutamate + D-Ala
-
 ?
pyroglutamyl-p-nitroanilide + H2O
-
 Gallus gallus pyroglutamate + p-nitroaniline
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.8, 15 min, stable Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 hydrolysis of pyroglutamyl-2-naphthylamide or pyroglutamyl-Ala Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 30°C, 30 min, stable in presence of 5 mM each of EDTA and 2-mercaptoethanol Gallus gallus
7 8.2 stable Gallus gallus