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Literature summary for 3.4.19.3 extracted from
- Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis (2000), Extremophiles, 4, 297-303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli under the control of an inducible tac promoter | Thermococcus litoralis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Thermococcus litoralis |  |
| N-ethylmaleimide | - |
Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24747 | - |
4 * 24747, calculated from sequence | Thermococcus litoralis |
| 80000 | 108000 | gel filtration | Thermococcus litoralis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitrile | at 50% (v/v) concentrations more than 50% of the initial activity is retained | Thermococcus litoralis |
| Ethanol | the enzyme retains stability at concentrations up to 90% (v/v) | Thermococcus litoralis |
| isopropanol | at 50% (v/v) concentrations more than 50% of the initial activity is retained | Thermococcus litoralis |
| Methanol | the enzyme retains stability at concentrations up to 75% (v/v) | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | O07883 | - |
- |
| Thermococcus litoralis DSM 5473 | O07883 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
pH 7.5, temperature not specified in the publication | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl L-pyroglutamate + H2O | - |
Thermococcus litoralis | L-pyroglutamate + ethanol | - |
? | |
| ethyl L-pyroglutamate + H2O | - |
Thermococcus litoralis DSM 5473 | L-pyroglutamate + ethanol | - |
? | |
| L-pyroglutamyl-beta-naphthylamide + H2O | - |
Thermococcus litoralis | L-pyroglutamate + 2-naphthylamine | - |
? | |
| L-pyroglutamyl-beta-naphthylamide + H2O | - |
Thermococcus litoralis DSM 5473 | L-pyroglutamate + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 24747, calculated from sequence | Thermococcus litoralis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCP | - |
Thermococcus litoralis |
| pyrrolidone carboxyl peptidase | - |
Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermococcus litoralis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 50% of maximal activity | Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life: approximately 1 h | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermococcus litoralis |
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