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Literature summary for 3.4.19.3 extracted from

  • Dando, P.M.; Fortunato, M.; Strand, G.B.; Smith, T.S.; Barrett, A.J.
    Pyroglutamyl-peptidase I: cloning, sequencing, and characterisation of the recombinant human enzyme (2003), Protein Expr. Purif., 28, 111-119.
    View publication on PubMed
Search for more literature for 3.4.19.3

Activating Compound

Activating Compound Comment Organism Structure
thio-reducing agent absolute requirement Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed by use of a baculovirus vector in Spodoptera frugiperda Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-Pyrrolidone reversible Homo sapiens
Ca2+ 1 mM, 20% inhibition Homo sapiens
Cu2+ 1 mM, 96% inhibition Homo sapiens
E64 0.1 mM, 15% inhibition Homo sapiens
NEM reversible Homo sapiens
Ni2+ 0.1 mM, 98% inhibition Homo sapiens
Zn2+ 1 mM, 98% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 pyroglutamyl-4-methylcoumaryl-7-amide pH 7.8, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23188
-
 1 * 23188, calculation from nucleotide sequence Homo sapiens
24000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NXJ5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colon mucosa Homo sapiens
-
mucosa from colon Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, retains more than 90% of its activity for 1 month in the presence of a thiol-reducing agent Homo sapiens
4°C, pH 7.8, stable for at least 1 week Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ACE inhibitor + H2O contains the N-terminal dipeptide pyroglutamyl-Trp- Homo sapiens pyroglutamate + ?
-
 ?
bombesin + H2O contains the N-terminal dipeptide pyroglutamyl-Arg- Homo sapiens pyroglutamate + ?
-
 ?
bradykinin potentiator B + H2O contains the N-terminal dipeptide pyroglutamyl-Gly- Homo sapiens pyroglutamate + ?
-
 ?
leukopyrokinin + H2O contains the N-terminal dipeptide pyroglutamyl-Thr- Homo sapiens pyroglutamate + ?
-
 ?
luteinizing hormone releasing hormone + H2O contains the N-terminal dipeptide pyroglutamyl-His- Homo sapiens pyroglutamate + ?
-
 ?
neurotensin + H2O contains the N-terminal dipeptide pyroglutamyl-Leu- Homo sapiens pyroglutamate + ?
-
 ?
pyroglutamyl-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens pyroglutamate + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23188, calculation from nucleotide sequence Homo sapiens

Synonyms

Synonyms Comment Organism
PGPEP1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 4.5 h, stable up to Homo sapiens
50
-
 90 min, enzyme is completely stable in crude preparation Homo sapiens
60
-
 30 min, activity is almost completely lost Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 75% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 NEM pH 7.8, 37°C Homo sapiens
0.05
-
 2-Pyrrolidone pH 7.8, 37°C Homo sapiens