Literature summary for 3.4.19.15 extracted from

Varga, S.; Pathare, G.R.; Baka, E.; Boicu, M.; Kriszt, B.; Szekacs, A.; Zinzula, L.; Kukolya, J.; Nagy, I.
Enhancing recombinant protein solubility with ubiquitin-like small archeal modifying protein fusion partners (2015), J. Microbiol. Methods, 118, 113-122 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Haloferax volcanii

Organism

Organism	UniProt	Comment	Textmining
Haloferax volcanii	D4GTS4	-	-
Haloferax volcanii ATCC 29605	D4GTS4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Haloferax volcanii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an N6-[small archaeal modifier protein]-[protein]-L-lysine + H2O	Hvo_2505 mixed with Ta0895_VSGG-Ta0547 results cleavage products at 18 kDa (Ta0547) and 14 kDa (Ta0895_VSGG). Hvo_2505 is active at 24°C, 37°C and 48°C cleaving a substantial percentage of Ta0895_VAGG-ScRpn11 and Ta0895_VSGG-Ta0547. No cleavage activity is detected at lower (10°C) temperature. Inactive on Ta1019-ScRpn11 and Mbur_1415-ScRpn11 fusion protein substrates	Haloferax volcanii	a [protein]-L-lysine + a small archaeal modifier protein	-	?
an N6-[small archaeal modifier protein]-[protein]-L-lysine + H2O	Hvo_2505 mixed with Ta0895_VSGG-Ta0547 results cleavage products at 18 kDa (Ta0547) and 14 kDa (Ta0895_VSGG). Hvo_2505 is active at 24°C, 37°C and 48°C cleaving a substantial percentage of Ta0895_VAGG-ScRpn11 and Ta0895_VSGG-Ta0547. No cleavage activity is detected at lower (10°C) temperature. Inactive on Ta1019-ScRpn11 and Mbur_1415-ScRpn11 fusion protein substrates	Haloferax volcanii ATCC 29605	a [protein]-L-lysine + a small archaeal modifier protein	-	?

Synonyms

Synonyms	Comment	Organism
HVO_2505	-	Haloferax volcanii
JAMM protease	-	Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
24	48	the enzyme is active at 24°C, 37°C and 48°C cleaving a substantial percentage of Ta0895_VAGG-ScRpn11 and Ta0895_VSGG-Ta0547, but no cleavage activity is detected at lower (10°C) temperature	Haloferax volcanii

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Release 2023.1 (January 2023)