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Literature summary for 3.4.19.12 extracted from
- Contribution of active site glutamine to rate enhancement in ubiquitin C-terminal hydrolases (2012), FEBS J., 279, 1106-1118.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q82A | kcat and Km can not be determined individually because, even at concentrations of ubiquitin 7-amido-4-methylcoumarin as high as 12 microM, the Michaelis-Menten plot is still rising linearly with substrate concentration, not reaching the plateau that is diagnostic of saturation | Homo sapiens |
| Q84A | Km (ubiquitin 7-amido-4-methylcoumarin) increased compared to wild-type, kcat (ubiquitin 7-amido-4-methylcoumarin) decreased | Homo sapiens |
| Q89A | Km (ubiquitin 7-amido-4-methylcoumarin) increased compared to wild-type, kcat (ubiquitin 7-amido-4-methylcoumarin) decreased | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000047 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 0.000056 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q84A | Homo sapiens | |
| 0.0000771 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 0.0000991 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q89A | Homo sapiens | |
| 0.02149 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P09936 | - |
- |
| Homo sapiens | P15374 | - |
- |
| Homo sapiens | Q9Y5K5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquitin 7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UCHL1 | - |
Homo sapiens |
| Uchl3 | - |
Homo sapiens |
| UCHL5N240 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q89A | Homo sapiens | |
| 0.0348 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 1.03 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q89A | Homo sapiens | |
| 18.6 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 33.67 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000104 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q89A | Homo sapiens | |
| 0.0002414 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 9.6 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q84A | Homo sapiens | |
| 19.6 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, mutant Q84A | Homo sapiens | |
| 157 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens | |
| 7410 | - |
ubiquitin 7-amido-4-methylcoumarin | pH 7.6, 30°C, wild-type | Homo sapiens |
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