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Literature summary for 3.4.19.12 extracted from

  • Enesa, K.; Zakkar, M.; Chaudhury, H.; Luong, l.e..A.; Rawlinson, L.; Mason, J.C.; Haskard, D.O.; Dean, J.L.; Evans, P.C.
    NF-kappaB suppression by the deubiquitinating enzyme Cezanne: a novel negative feedback loop in pro-inflammatory signaling (2008), J. Biol. Chem., 283, 7036-7045.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
TNFalpha inducer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression vectors encoding HA epitope-tagged Cezanne (pHM6-Cezanne), GFP-tagged Cezanne (pEGFP-Cezanne) and enzymatically inactive forms mutated at the catalytic cysteine (pHM6-Cezanne C209S and pEGFP-Cezanne C209S). Expressed in HEK293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C209S catalytically inactive, does not suppress NF-kappaB nuclear translocation and transcriptional activity by targeting the TNFR signaling pathway at the level of the IkappaB kinase complex or upstream from it. Does not suppress polyubiquitinated RIP1 and does not enhance the levels of monubiquinated or unmodified RIP1 as compared to the wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HUVEC cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Cezanne-specific siRNA suppresses Cezanne mRNA and protein by at least 70% in culture cells. It leads to elevated NF-kappaB luciferase reporter gene activity and enhanced expression of IL-8 transcripts in TNFalpha-treated cells. Endogenous Cezanne can attenuate NF-kappaB activation and the induction of pro-inflammatory transcripts in response to TNF receptor signaling. Deubiquitinating activity of Cezanne is essential for NF-kappB suppression. Cezanne can be recruited to activated TNF receptors where it suppresses the build-up of polyubiquitinated RIP1 signal adapter proteins Homo sapiens ?
-
?
polyubiquitin + H2O Cezanne deubiquitinates Lys48- or Lys63-polyubiquitinated cellular proteins Homo sapiens ubiquitin
-
?

Synonyms

Synonyms Comment Organism
A20
-
Homo sapiens
Cezanne
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Homo sapiens