Cloned (Comment) | Organism |
---|---|
gene uchl1, phylogenetic analysis | Monopterus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
Western blot analysis | Monopterus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Monopterus albus | Uch-L1, via the ubiquitin-proteasome system, may play an important role not only in gametogenesis, but also in the gonadal transformation process in the rice field eel | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monopterus albus | B5KNK6 | - |
- |
Monopterus albus | B5KNK6 | gene uchl1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | slight expression | Monopterus albus | - |
brain | expression of both mRNA and protein | Monopterus albus | - |
gonad | in male gonad epithelium | Monopterus albus | - |
additional information | Uch-L1 is upregulated during gonadal transformation, especially from the beginning of the intersex stage onwards | Monopterus albus | - |
additional information | no expression in heart, liver, kidney, or spleen. Uch-L1 is upregulated during gonadal transformation from female to male, overview | Monopterus albus | - |
ovary | expression of both mRNA and protein mainly in the developing ovary and slightly in the mature ovary | Monopterus albus | - |
ovary | slight expression, in degraded ovaries | Monopterus albus | - |
ovotestis | during the intersex stage, both mRNA and protein is expressed in the male gonad epithelium and degraded ovary | Monopterus albus | - |
ovotestis | dominant expression | Monopterus albus | - |
spermatocyte | - |
Monopterus albus | - |
spermatogonium | - |
Monopterus albus | - |
testis | expression of both mRNA and protein in developing germ cells | Monopterus albus | - |
testis | dominant expression | Monopterus albus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Uch-L1, via the ubiquitin-proteasome system, may play an important role not only in gametogenesis, but also in the gonadal transformation process in the rice field eel | Monopterus albus | ? | - |
? | |
additional information | Uch-L1 binds to and interacts with monoubiqutin | Monopterus albus | ? | - |
? | |
polyubiquitin + H2O | - |
Monopterus albus | ubiquitin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE, Uch-L1 undergoes dimerization and oligomerization in gonads, whereby the relative level of dimerization/oligomerization decreases during gonadal transformation. Rates of dimerization and oligomerization are high in ovary and lower in ovotestis, whereas monomers are mainly formed in testis | Monopterus albus |
dimer | 2 * 26000, SDS-PAGE, rates of dimerization and oligomerization are high in ovary and lower in ovotestis, whereas monomers are mainly formed in testis. The dimerization/oligomerization level of Uch-L1 decreases during gonadal transformation from female to male. UCH-L1 structure modelling | Monopterus albus |
monomer | 1 * 26000, SDS-PAGE, rates of dimerization and oligomerization are high in ovary and lower in ovotestis, whereas monomers are mainly formed in testis. The dimerization/oligomerization level of Uch-L1 decreases during gonadal transformation from female to male. UCH-L1 structure modelling | Monopterus albus |
Synonyms | Comment | Organism |
---|---|---|
More | Uch-L1 is a member of the Uch family | Monopterus albus |
ubiquitin C-terminal hydrolase-L1 | - |
Monopterus albus |
ubiquitin carboxyl-terminal hydrolase-L1 | - |
Monopterus albus |
UCH-L1 | - |
Monopterus albus |
General Information | Comment | Organism |
---|---|---|
physiological function | Uch-L1 plays an important role in the maintenance of intracellular monoubiquitin levels by generating ubiquitin through the hydrolysis of polyubiquitinated proteins, and regulates the ubiquitin pathway. It is important in gametogenesis and gonadal transformation | Monopterus albus |