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Literature summary for 3.4.19.12 extracted from

  • Meray, R.K.; Lansbury, P.T.
    Reversible monoubiquitination regulates the Parkinson disease-associated ubiquitin hydrolase UCH-L1 (2007), J. Biol. Chem., 282, 10567-10575.
    View publication on PubMed

Application

Application Comment Organism
medicine Parkinson disease-associated UCH-L1 is regulated by reversible monoubiquitination involving auto-deubiquitination Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
COS-7 cells co-transfected with plasmids encoding His-tagged UCH-L1 and FLAG-tagged ubiquitin Homo sapiens

Protein Variants

Protein Variants Comment Organism
C90S increases cellular monoubiquitin levels, 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated Homo sapiens
C90S/K157R reduces monoubiquitination Homo sapiens
D30K deficient in ubiquitin binding, is unable to increase cellular monoubiquitin levels Homo sapiens
H161D 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated Homo sapiens
I93M approximately doubles the monoubiquitination level of UCH-L1 Homo sapiens
additional information fusion protein UbVV/L8A-UCH-L1 contains an L8A mutation in the ubiquitin moiety. Efficiently binds ubiquitin, increases cellular monoubiquitin levels Homo sapiens
S18Y does not affect cellular levels of monoubiquitinated UCH-L1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide prevents deubiquitination Homo sapiens
additional information monoubiquitination inhibits ubiquitin binding in vitro and prevents UCH-L1 from regulating free ubiquitin levels in cells involving auto-deubiquitination. Intramolecular deubiquitination, which is dependent on the hydrolytic activity of UCH-L1, regulates the lifetime of monoubiquitination, thereby affecting the cellular level of monoubiquitinated UCH-L1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-
additional information perinucleus Homo sapiens
-
-
nucleus
-
Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA resin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquitin + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ubiquitin C-terminal hydrolase-L1
-
Homo sapiens
UCH-L1
-
Homo sapiens