Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.12 extracted from

  • Moskovitz, J.
    Characterization of the 30-kDa enzyme from red blood cells that cleaves ubiquitin-protein conjugates (1994), Biochem. Biophys. Res. Commun., 205, 354-360.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Bos taurus
iodoacetamide
-
Homo sapiens
Ubiquitin aldehyde
-
Bos taurus
ubiquitin-aldehyde
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
erythrocyte
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.75
-
purified enzyme, substrate A24 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
conjugate of histone H2A and ubiquitin + H2O best substrate Bos taurus ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds Bos taurus ubiquitin + histone H2A
-
?
additional information enzyme contains ubiquitin-C-terminal hydrolase activity towards biosynthetic precursors of ubiquitin as well as isopeptidase activity towards ubiquitin histone conjugates as human ubiquitin carboxyl extension protein and ubiquitin C-terminal amide Bos taurus ?
-
?
ubiquitin C-terminal amide + H2O
-
Homo sapiens ubiquitin + NH3
-
?
ubiquitin fused to 60% of its N-terminus + H2O recombinant substrate, expressed in Escherichia coli Bos taurus ubiquitin + 60% of ubiquitin N-terminus
-
?
ubiquitin metallothionein + H2O low activity Bos taurus ?
-
?
ubiquitin metallothionein + H2O recombinant substrate, expressed in Escherichia coli Bos taurus ?
-
?
ubiquitin metallothionein + H2O i.e. UbMT Bos taurus ?
-
?
ubiquitin-carboxyl extension protein + H2O carboxyl extension protein of 52 amino acids Homo sapiens ubiquitin + ubiquitin-carboxyl extension protein
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O i.e. HUbCEP-52 Bos taurus ubiquitin + human carboxyl extension protein
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O recombinant substrate, expressed in Escherichia coli Bos taurus ubiquitin + human carboxyl extension protein
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
60 min, 70% loss of activity towards A24, no loss of activity towards ubiquitin C-terminal amide and human ubiquitin carboxyl extension protein Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Bos taurus