BRENDA - Reference to 3.4.19.1; Id = 95251

analysis

enzyme is useful for deblocking of peptides

Rattus norvegicus

75% loss of activity in 0.1 M guanidine hydrochloride at 37°C after 200 min

Rattus norvegicus

1-ethyl-3,3'-dimethylaminopropylcarbodiimide

-

Rattus norvegicus

5,5'-dithiobis-(2-nitrobenzoate)

partial

Rattus norvegicus

Cl-

activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala

Rattus norvegicus

DFP

-

Rattus norvegicus

diethyl dicarbonate

-

Rattus norvegicus

Gly methyl ester

-

Rattus norvegicus

iodoacetamide

partial

Rattus norvegicus

iodoacetic acid

partial

Rattus norvegicus

N-acetyl-Ala

competitive

Rattus norvegicus

N-acetyl-Ala chloromethyl ketone

inactivation follows first order kinetics, acetyl-Ala protects

Rattus norvegicus

N-ethyl-5-phenylisoxazolium 3'-sulfonate

i.e. Woodward's reagent

Rattus norvegicus

SCN-

activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala

Rattus norvegicus

Zn2+

most potent inhibitor

Rattus norvegicus

Cl-

0.1-0.5 M, activates activity towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide

Rattus norvegicus

SCN-

0.1-0.5 M, activates towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide

Rattus norvegicus

290000

320000

gel filtration

Rattus norvegicus

-

liver

-

Rattus norvegicus

-

N-acetyl-Ala p-nitroanilide + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + p-nitroaniline

-

?

N-acetyl-Ala-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ala

-

?

N-acetyl-Ala-Ala-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ala-Ala

-

?

N-acetyl-Ala-Asp + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Asp

-

?

N-acetyl-Ala-beta-naphthylamide + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + beta-naphthylamine

-

?

N-acetyl-Ala-Gly + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Gly

-

?

N-acetyl-Ala-Leu + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Leu

-

?

N-acetyl-Ala-Met + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Met

-

?

N-acetyl-Ala-Phe + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Phe

-

?

N-acetyl-Ala-Ser + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ser

-

?

N-acetyl-Ala-Thr + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Thr

-

?

N-acetyl-Ala-Trp + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Trp

-

?

N-acetyl-Ala-Tyr + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Tyr

-

?

N-acetyl-Gly-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Gly + Ala

-

?

N-acetyl-Leu-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Leu + Ala

-

?

N-acetyl-Met-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Met + Ala

-

?

N-acetyl-Phe-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Phe + Ala

-

?

N-acetyl-Ser-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ser + Ala

-

?

N-acetyl-Tyr-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Tyr + Ala

-

?

7.5

-

hydrolysis of N-acetyl-Ala p-nitroanilide

Rattus norvegicus

analysis

enzyme is useful for deblocking of peptides

Rattus norvegicus

75% loss of activity in 0.1 M guanidine hydrochloride at 37°C after 200 min

Rattus norvegicus

1-ethyl-3,3'-dimethylaminopropylcarbodiimide

-

Rattus norvegicus

5,5'-dithiobis-(2-nitrobenzoate)

partial

Rattus norvegicus

Cl-

activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala

Rattus norvegicus

DFP

-

Rattus norvegicus

diethyl dicarbonate

-

Rattus norvegicus

Gly methyl ester

-

Rattus norvegicus

iodoacetamide

partial

Rattus norvegicus

iodoacetic acid

partial

Rattus norvegicus

N-acetyl-Ala

competitive

Rattus norvegicus

N-acetyl-Ala chloromethyl ketone

inactivation follows first order kinetics, acetyl-Ala protects

Rattus norvegicus

N-ethyl-5-phenylisoxazolium 3'-sulfonate

i.e. Woodward's reagent

Rattus norvegicus

SCN-

activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala

Rattus norvegicus

Zn2+

most potent inhibitor

Rattus norvegicus

Cl-

0.1-0.5 M, activates activity towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide

Rattus norvegicus

SCN-

0.1-0.5 M, activates towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide

Rattus norvegicus

290000

320000

gel filtration

Rattus norvegicus

liver

-

Rattus norvegicus

-

N-acetyl-Ala p-nitroanilide + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + p-nitroaniline

-

?

N-acetyl-Ala-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ala

-

?

N-acetyl-Ala-Ala-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ala-Ala

-

?

N-acetyl-Ala-Asp + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Asp

-

?

N-acetyl-Ala-beta-naphthylamide + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + beta-naphthylamine

-

?

N-acetyl-Ala-Gly + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Gly

-

?

N-acetyl-Ala-Leu + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Leu

-

?

N-acetyl-Ala-Met + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Met

-

?

N-acetyl-Ala-Phe + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Phe

-

?

N-acetyl-Ala-Ser + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Ser

-

?

N-acetyl-Ala-Thr + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Thr

-

?

N-acetyl-Ala-Trp + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Trp

-

?

N-acetyl-Ala-Tyr + H2O

-

95251

Rattus norvegicus

N-acetyl-Ala + Tyr

-

?

N-acetyl-Gly-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Gly + Ala

-

?

N-acetyl-Leu-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Leu + Ala

-

?

N-acetyl-Met-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Met + Ala

-

?

N-acetyl-Phe-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Phe + Ala

-

?

N-acetyl-Ser-Ala + H2O

-

95251

Rattus norvegicus

N-acetyl-Ser + Ala

-

?

N-acetyl-Tyr-Ala + H2O

weak activity

95251

Rattus norvegicus

N-acetyl-Tyr + Ala

-

?

7.5

-

hydrolysis of N-acetyl-Ala p-nitroanilide

Rattus norvegicus

731083

Are

Expression, purification, crys ...

Deinococcus radiodurans

Acta Crystallogr. Sect. F

70

1292-1295

2014

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

731358

Papaleo

Structural investigation of th ...

Deinococcus radiodurans

Biochim. Biophys. Acta

1844

2203-2213

2014

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

731801

Gogliettino

A novel class of bifunctional ...

Trematomus bernacchii

FEBS J.

281

401-415

2014

-

2

-

3

-

4

-

9

-

5

1

-

1

3

5

1

-

2

-

4

-

18

-

5

2

-

2

6

5

2

-

1

-

2

732175

Santhoshkumar

Lens crystallin modifications ...

Mus musculus

J. Biol. Chem.

289

9039-9052

2014

-

4

-

1

-

1

-

1

-

1

-

2

-

725525

Menyhard

A self-compartmentalizing hexa ...

Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428

J. Biol. Chem.

288

17884-17894

2013

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

720867

Gogliettino

Identification and characteris ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

PLoS One

7

e37921

2012

-

4

6

1

-

3

-

1

5

-

1

-

2

-

16

1

2

3

6

1

2

-

1

-

4

1

6

1

-

3

-

1

-

1

-

2

-

16

1

2

3

6

1

2

-

1

-

1

9

723816

Zhou

Alteration of substrate specif ...

Aeropyrum pernix

Acta Biochim. Biophys. Sin.

44

965-973

2012

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

724738

Li

-

Expression and characterizatio ...

Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7

Chem. Res. Chin. Univ.

28

851-855

2012

-

1

-

6

-

2

-

1

-

11

-

1

-

2

-

1

-

1

-

6

-

1

-

11

-

1

-

2

-

1

-

728169

Papaleo

Coupled motions during dynamic ...

Aeropyrum pernix, Aeropyrum pernix DSM 11879

J. Mol. Graph. Model.

38

226-234

2012

-

1

-

5

-

2

-

1

-

2

-

732576

Sandoval

Synaptic localization of acylp ...

Rattus norvegicus

Neurosci. Lett.

520

98-103

2012

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

732661

Nakai

Role of acylamino acid-releasi ...

Arabidopsis thaliana

Planta

236

427-436

2012

-

2

-

3

-

2

-

2

-

732692

Papaleo

Mechanisms of intramolecular c ...

Sporosarcina psychrophila

PLoS ONE

7

e35686

2012

-

5

-

2

-

1

-

1

-

5

-

2

-

1

-

726281

Palmieri

Acylpeptide hydrolase inhibiti ...

Saccharolobus solfataricus

PLoS One

6

e25888

2011

-

2

-

2

-

1

-

2

-

2

-

2

-

1

-

727876

Harmat

Structure and catalysis of acy ...

Aeropyrum pernix, Aeropyrum pernix DSM 11879

J. Biol. Chem.

286

1987-1998

2011

-

1

2

-

4

-

1

-

6

-

4

1

-

4

2

1

-

1

2

-

4

-

1

-

4

1

-

4

2

1

-

6

728716

Liu

Switch of substrate specificit ...

Aeropyrum pernix, Aeropyrum pernix DSM 11879

Protein Cell

2

497-506

2011

-

6

-

7

-

2

-

6

-

2

-

720431

Palmieri

A novel class of protease targ ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

Mol. Biosyst.

6

2498-2507

2010

-

1

-

3

2

1

-

2

-

6

-

1

-

1

-

6

1

-

2

1

-

1

-

1

-

1

-

1

3

1

2

1

-

2

-

1

-

1

-

6

1

-

2

1

-

2

696454

Szeltner

Characterization of a novel ac ...

Pyrococcus horikoshii

Biochim. Biophys. Acta

1794

1204-1210

2009

-

1

-

1

-

3

-

1

-

11

1

-

1

-

1

-

1

-

1

-

11

1

-

1

-

696472

Yang

Glu88 in the non-catalytic dom ...

Aeropyrum pernix K1

Biochim. Biophys. Acta

1794

94-102

2009

-

1

-

5

-

7

-

1

-

2

-

1

-

2

-

2

-

7

1

-

1

-

5

-

7

-

1

-

1

-

2

-

2

-

7

1

-

710615

Olmos

Effect of short-term exposure ...

Rattus norvegicus

Toxicol. Appl. Pharmacol.

238

37-46

2009

-

1

-

2

-

2

-

1

-

1

-

2

-

1

-

688698

Kiss

Structural and kinetic contrib ...

Aeropyrum pernix, Aeropyrum pernix K1

J. Struct. Biol.

162

312-323

2008

-

1

2

-

2

4

-

1

-

3

-

1

-

2

1

2

-

1

-

1

-

4

-

1

-

2

-

2

4

-

1

-

1

-

2

1

2

-

1

-

1

-

696409

Zhang

The conserved N-terminal helix ...

Aeropyrum pernix K1

Biochim. Biophys. Acta

1784

1176-1183

2008

-

1

-

4

-

5

-

1

-

3

-

1

-

2

-

3

-

5

1

-

1

-

4

-

5

-

1

-

1

-

2

-

3

-

5

1

-

696524

Boehme

Isoaspartate residues dramatic ...

Sus scrofa

Biol. Chem.

389

1043-1053

2008

-

1

-

1

-

5

-

1

-

1

-

1

-

5

-

1

-

1

-

688351

Kiss

The acylaminoacyl peptidase fr ...

Aeropyrum pernix K1

J. Mol. Biol.

368

509-520

2007

-

1

-

2

-

2

-

13

1

-

1

-

1

-

2

-

13

1

-

688494

Yamin

Acyl peptide hydrolase, a seri ...

Homo sapiens

J. Neurochem.

100

458-467

2007

-

1

-

1

-

7

-

2

-

1

-

3

-

2

-

1

-

1

-

7

-

1

-

3

-

2

-

688712

Pancetti

Noncholinesterase effects indu ...

Homo sapiens, Sus scrofa

J. Toxicol. Environ. Health B Crit. Rev.

10

623-630

2007

-

2

-

5

-

3

-

2

-

1

-

2

-

5

-

2

-

1

-

669473

Wang

Discrimination of esterase and ...

Aeropyrum pernix

J. Biol. Chem.

281

18618-18625

2006

-

6

-

13

-

2

-

2

-

14

-

6

-

13

-

2

-

14

-

680739

Edosada

Selective inhibition of fibrob ...

synthetic construct

J. Biol. Chem.

281

7437-7444

2006

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

687846

Nishimura

Molecular cloning and characte ...

Streptomyces morookaense, Streptomyces morookaense JCM 4673

J. Biosci. Bioeng.

101

63-69

2006

1

-

6

-

6

-

1

-

7

1

15

-

6

-

1

-

6

-

1

-

7

1

15

-

6

-

667028

Zhang

Expression, purification and c ...

Aeropyrum pernix K1

Acta Biochim. Biophys. Sin.

37

613-617

2005

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

2

-

667093

Wright

Crystallization and preliminar ...

Sus scrofa

Acta Crystallogr. Sect. F

F61

942-944

2005

-

1

-

2

-

1

-

1

-

1

-

667841

Perrier

Catabolism of intracellular N- ...

Homo sapiens, Rattus norvegicus, Sus scrofa

Biochimie

87

673-685

2005

-

3

-

13

-

13

-

670956

Quistad

Blood acylpeptide hydrolase ac ...

Homo sapiens, Mus musculus

Toxicol. Sci.

86

291-299

2005

-

2

-

51

-

7

-

2

-

2

-

51

-

2

-

51

-

2

-

2

-

668593

Kiss

His507 of acylaminoacyl peptid ...

Sus scrofa

FEBS Lett.

571

17-20

2004

-

1

-

1

-

1

-

1

-

1

-

670903

Bartlam

Crystal structure of an acylpe ...

Aeropyrum pernix

Structure

12

1481-1488

2004

-

1

-

5

-

1

-

1

-

1

-

651807

Yamauchi

Identification and biochemical ...

Arabidopsis thaliana, Cucumis sativus

J. Biochem.

134

251-257

2003

-

2

-

12

2

-

4

1

-

7

-

2

-

3

1

-

29

2

-

2

-

1

-

2

-

12

-

2

-

4

1

-

2

-

3

1

-

29

2

-

2

-

1

-

653041

Durand

Structural properties of porci ...

Sus scrofa

J. Protein Chem.

22

183-191

2003

-

1

-

1

-

2

-

1

-

3

-

1

-

1

-

1

-

1

-

3

-

1

-

649173

Wang

Crystallization and preliminar ...

Aeropyrum pernix

Acta Crystallogr. Sect. D

58

1054-1055

2002

-

1

-

1

-

1

-

653037

Senthilkumar

Effect of chaotropic agents on ...

Sus scrofa

J. Protein Chem.

21

323-332

2002

-

2

-

1

-

2

-

1

-

1

-

1

2

-

1

-

2

-

1

-

2

-

1

-

1

2

-

1

-

653281

Richards

Identification of acylpeptide ...

Sus scrofa

Mol. Pharmacol.

58

577-583

2000

-

8

-

1

-

1

-

3

-

1

-

1

-

2

-

8

-

8

-

1

-

1

-

1

-

1

-

2

-

95255

Scaloni

Structural investigations on h ...

Homo sapiens

J. Protein Chem.

18

349-360

1999

-

1

-

2

-

2

-

2

-

1

-

2

-

2

-

95259

Raphel

Cloning, sequencing and furthe ...

Sus scrofa

Biochim. Biophys. Acta

1432

371-381

1999

-

1

-

5

4

-

2

-

3

-

1

-

2

-

4

1

-

2

-

1

-

5

-

4

-

2

-

1

-

2

-

4

1

-

2

-

95261

Ishikawa

Acylamino acid-releasing enzym ...

Pyrococcus horikoshii

J. Biol. Chem.

273

17726-17731

1998

-

1

-

4

-

1

-

6

-

14

-

2

-

2

4

2

1

-

1

-

4

-

1

-

14

-

2

-

2

4

2

1

-

95268

Chongcharoen

Characterization of trypsin: m ...

Bos taurus

Biochem. Biophys. Res. Commun.

247

136-141

1998

-

2

-

1

-

2

1

-

3

-

1

-

1

-

2

1

-

3

-

1

-

95271

Mitta

Identification of the catalyti ...

Sus scrofa

J. Biochem.

123

924-931

1998

-

1

-

3

-

3

-

3

-

2

-

1

-

3

-

1

-

3

-

3

-

2

-

1

-

3

-

745261

Ishikawa

Acylamino acid-releasing enzy ...

Pyrococcus horikoshii

J. Biol. Chem.

273

17726-17731

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-

1

-

8

-

1

-

6

-

1

-

29

-

15

1

3

-

1

8

2

-

1

-

8

-

1

-

1

-

29

-

15

1

3

-

1

8

2

-

1

-

95265

Sharma

Peptide hydrolysis in lens: ro ...

Bos taurus

Curr. Eye Res.

15

363-369

1996

-

1

-

1

-

2

-

1

-

2

-

95272

Miyagi

Complete covalent structure of ...

Sus scrofa

J. Biochem.

118

771-779

1995

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

95256

Jones

Acylaminoacyl-peptidase ...

Homo sapiens

Methods Enzymol.

244

227-231

1994

-

3

-

1

-

1

-

1

-

3

-

2

-

3

-

1

-

1

-

3

-

2

-

95264

Scaloni

Human acylpeptide hydrolase. S ...

Homo sapiens

J. Biol. Chem.

269

15076-15084

1994

-

3

7

-

2

-

8

-

3

-

7

-

8

-

95263

Sharma

Bovine lens acylpeptide hydrol ...

Bos taurus

Eur. J. Biochem.

216

631-637

1993

-

1

10

-

2

-

3

-

1

-

2

-

1

-

1

-

1

-

10

-

2

-

1

-

2

-

1

-

1

-

95270

Raphel

The N-acylpeptide hydrolase fr ...

Sus scrofa

Biochimie

75

891-897

1993

-

1

-

1

-

2

-

1

-

5

1

-

5

-

1

-

1

-

1

-

5

1

-

5

-

95273

Feese

Crystallization and preliminar ...

Homo sapiens

J. Mol. Biol.

233

546-549

1993

-

1

-

2

-

2

-

1

-

2

-

29061

Sherriff

Isolation and purification of ...

Rattus norvegicus

Biochim. Biophys. Acta

1119

275-280

1992

-

3

9

-

1

-

1

-

1

-

1

-

10

1

-

9

1

-

3

-

9

-

1

-

1

-

1

-

10

1

-

9

1

-

95262

Scaloni

Acylpeptide hydrolase: inhibit ...

Homo sapiens

J. Biol. Chem.

267

3811-3818

1992

-

3

-

1

-

2

-

1

-

3

-

1

-

1

-

95269

Krishna

Specificity determinants of ac ...

Oryctolagus cuniculus

Protein Sci.

1

582-589

1992

-

4

-

2

-

2

-

17

-

1

-

4

-

2

-

17

-

1

-

95258

Farries

Enzyme-mediated peptide synthe ...

Ovis aries

Eur. J. Biochem.

196

687-692

1991

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

95260

Farries

Removal of N-acetyl groups fro ...

Ovis aries

Eur. J. Biochem.

196

679-685

1991

-

1

-

1

9

-

1

-

1

-

1

-

1

-

1

-

1

-

9

-

1

-

1

-

1

-

95266

Jones

Genetic relationship between a ...

Homo sapiens

Proc. Natl. Acad. Sci. USA

88

2194-2198

1991

-

2

-

1

-

8

-

1

-

8

-

95267

Radhakrishna

Purification and characterizat ...

Oryctolagus cuniculus

J. Biol. Chem.

264

11076-11081

1989

-

7

-

1

-

1

-

2

-

1

-

2

1

2

1

-

2

-

7

-

1

-

1

-

1

-

2

1

2

1

-

2

-

95251

Kobayashi

Acyl-peptide hydrolase from ra ...

Rattus norvegicus

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262

11435-11445

1987

-

1

-

1

13

-

2

1

-

2

-

2

-

19

-

1

-

1

-

1

-

13

-

2

1

-

2

-

19

-

1

-

95252

Tsunasawa

Apparent dipeptidyl peptidase ...

Sus scrofa

J. Biochem.

93

1217-1220

1983

-

4

-

1

-

2

-

4

-

1

-

2

-

4

-

95253

Schoenberger

N-Acetylalanine aminopeptidase ...

Homo sapiens

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362

865-873

1981

-

3

2

1

-

1

-

1

-

1

-

1

-

6

-

2

1

3

-

3

-

2

-

1

-

1

-

1

-

1

-

6

-

2

1

3

-

95254

Tsunasawa

Purification and properties of ...

Escherichia coli, Escherichia coli B / ATCC 11303, Gallus gallus, Oryctolagus cuniculus, Rattus norvegicus

J. Biochem.

77

89-102

1975

-

1

-

1

8

-

3

-

181

-

1

-

13

2

-

1

-

1

-

1

-

1

-

8

-

3

-

1

-

13

2

-

1

-

1

-

Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction

Data extracted from this reference: